Seroatlas · Human Serome Atlas

DNAAF8

Dynein axonemal assembly factor 8

Also known as: C16orf71, DAAF8_HUMAN, DKFZp686H2240, FLJ43261

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IYS4
Gene: DNAAF8
Ensembl: ENSG00000166246
Chromosome: 16
Canonical length: 520 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable dynein complex binding activity. Predicted to be involved in outer dynein arm assembly. Located in dynein axonemal particle. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

520 residues, UniProt reviewed canonical sequence.

>Q8IYS4|DNAAF8
     1  MASNDKGMAP SLGSPWASQM GPWDAILKAV KDQLPSLDSD SPLSDYGEEE LFIFQRNQTS
    61  LIPDLSEELA EDPADGDKSR AWVAAAEESL PEPVLVPAEL ATEPGCRQNT RTKDASSQEG
   121  RDPGRPFESS GEVSALLGMA EEPPRWLEGD LGSLSFNTKG SQGPPWDPQA EATLSCHEGD
   181  PKAEPLSTAS QESVNRRALR QERRKMIETD ILQKVTRDAC GPTSSDKGGV KEAPCHAAES
   241  APRSKMPLVE PPEGPPVLSL QQLEAWDLDD ILQSLAGQED NQGNRAPGTV WWAADHRQVQ
   301  DRMVPSAHNR LMEQLALLCT TQSKASACAR KVPADTPQDT KEADSGSRCA SRKQGSQAGP
   361  GPQLAQGMRL NAESPTIFID LRQMELPDHL SPESSSHSSS DSEEEEEEEM AALGDAEGAS
   421  PSSLGLRTCT GKSQLLQQLR AFQKGTAQPE LPASKGPAGG RAQAPEDTAG SRTGRKQHMK
   481  LCAKGQSAQA RLPRGRPRAL GDVPEPGAAR EALMPPLEQL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAAF8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.68
Highest tissue expression: 74 nTPM

Expression across tissuesHPA

Tissue

testis: 74 nTPM
fallopian tube: 21 nTPM
choroid plexus: 11 nTPM
pituitary gland: 3.9 nTPM
hypothalamus: 2.7 nTPM
lung: 2.7 nTPM

Single-cell type

late primary spermatocytes: 242 nCPM
late spermatids: 99 nCPM
respiratory ciliated cells: 68 nCPM
fallopian tube ciliated cells: 50 nCPM
early primary spermatocytes: 45 nCPM
ependymal cells: 39 nCPM

Immune cell

plasmacytoid DC: 1.1 nTPM
naive B-cell: 0.4 nTPM
non-classical monocyte: 0.3 nTPM
classical monocyte: 0.2 nTPM
myeloid DC: 0.2 nTPM
memory CD4 T-cell: 0.1 nTPM

Brain region

choroid plexus: 22 nTPM
midbrain: 15 nTPM
medulla oblongata: 13 nTPM
spinal cord: 9.5 nTPM
pons: 7.2 nTPM
cerebellum: 7.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.52
gnomAD pLI: 0
DepMap mean gene effect: -0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

outer dynein arm assembly

Molecular functions

dynein complex binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Dynein axonemal assembly factor 8
Dynein axonemal-associated protein 1

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAAF8 as an antibody target. Whether an autoantibody or antibody against DNAAF8 could matter depends on whether native DNAAF8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAAF8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAAF8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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