Seroatlas · Human Serome Atlas

DHFR2

Dihydrofolate reductase 2, mitochondrial

Also known as: DHFRL1, DHFRP4, DYR2_HUMAN, FLJ16119

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86XF0
Gene: DHFR2
Ensembl: ENSG00000178700
Chromosome: 3
Canonical length: 187 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Mitochondria

OverviewNCBI Gene

Enables dihydrofolate reductase activity and mRNA binding activity. Involved in tetrahydrofolate metabolic process and thymidine biosynthetic process. Located in mitochondrial inner membrane and mitochondrial matrix. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

187 residues, UniProt reviewed canonical sequence.

>Q86XF0|DHFR2
     1  MFLLLNCIVA VSQNMGIGKN GDLPRPPLRN EFRYFQRMTT TSSVEGKQNL VIMGRKTWFS
    61  IPEKNRPLKD RINLVLSREL KEPPQGAHFL ARSLDDALKL TERPELANKV DMIWIVGGSS
   121  VYKEAMNHLG HLKLFVTRIM QDFESDTFFS EIDLEKYKLL PEYPGVLSDV QEGKHIKYKF
   181  EVCEKDD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DHFR2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 8 nTPM

Expression across tissuesHPA

Tissue

ovary: 8 nTPM
endometrium: 7.3 nTPM
liver: 6.2 nTPM
thyroid gland: 6.2 nTPM
parathyroid gland: 6.1 nTPM
cerebral cortex: 6 nTPM

Single-cell type

epicardial cells: 46 nCPM
cardiomyocytes: 43 nCPM
late primary spermatocytes: 41 nCPM
tuft cells: 35 nCPM
early spermatids: 28 nCPM
epididymal principal cells: 28 nCPM

Immune cell

MAIT T-cell: 3.3 nTPM
naive CD4 T-cell: 3.3 nTPM
T-reg: 3.2 nTPM
naive CD8 T-cell: 3 nTPM
memory CD8 T-cell: 2.9 nTPM
memory CD4 T-cell: 2.4 nTPM

Brain region

white matter: 16 nTPM
cerebellum: 15 nTPM
basal ganglia: 14 nTPM
cerebral cortex: 14 nTPM
pons: 14 nTPM
choroid plexus: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.44
gnomAD pLI: 0.22
DepMap mean gene effect: -0.46
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DHFR2 as an antibody target. Whether an autoantibody or antibody against DHFR2 could matter depends on whether native DHFR2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DHFR2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DHFR2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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