DENND6B
Protein DENND6B
Also known as: AFI1B, DEN6B_HUMAN, FAM116B, MGC33692
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q8NEG7
- Gene
- DENND6B
- Ensembl
- ENSG00000205593
- Chromosome
- 22
- Canonical length
- 585 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Plasma membrane
OverviewNCBI Gene
Enables guanyl-nucleotide exchange factor activity. Predicted to be located in cytosol. Predicted to be active in recycling endosome. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
585 residues, UniProt reviewed canonical sequence.
>Q8NEG7|DENND6B
1 MDALLGTGPR RARGCLGAAG PTSSGRAART PAAPWARFSA WLECVCVVTF DLELGQALEL
61 VYPNDFRLTD KEKSSICYLS FPDSHSGCLG DTQFSFRMRQ CGGQRSPWHA DDRHYNSRAP
121 VALQREPAHY FGYVYFRQVK DSSVKRGYFQ KSLVLVSRLP FVRLFQALLS LIAPEYFDKL
181 APCLEAVCSE IDQWPAPAPG QTLNLPVMGV VVQVRIPSRV DKSESSPPKQ FDQENLLPAP
241 VVLASVHELD LFRCFRPVLT HMQTLWELML LGEPLLVLAP SPDVSSEMVL ALTSCLQPLR
301 FCCDFRPYFT IHDSEFKEFT TRTQAPPNVV LGVTNPFFIK TLQHWPHILR VGEPKMSGDL
361 PKQVKLKKPS RLKTLDTKPG LYTAYTAHLH RDKALLKRLL KGVQKKRPSD VQSALLRRHL
421 LELTQSFIIP LEHYMASLMP LQKSITPWKT PPQIQPFSQD DFLRSLEHAG PQLTCILKGD
481 WLGLYRRFFK SPHFDGWYRQ RHKEMALKLE ALHLEAICEA NIETWMKDKS EVEVVDLVLK
541 LREKLVRAQG HQLPVKEATL QRAQLYIETV IGSLPKDLQA VLCPPLocalizationUniProt · AlphaFold · HPA
Whether an antibody against DENND6B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.29
- Highest tissue expression
- 25 nTPM
Expression across tissuesHPA
Tissue
- cerebellum: 25 nTPM
- cerebral cortex: 18 nTPM
- basal ganglia: 14 nTPM
- pituitary gland: 13 nTPM
- fallopian tube: 13 nTPM
- hypothalamus: 12 nTPM
Single-cell type
- megakaryocytes: 192 nCPM
- respiratory ciliated cells: 181 nCPM
- late spermatids: 129 nCPM
- ependymal cells: 115 nCPM
- fallopian tube ciliated cells: 101 nCPM
- epididymal efferent duct ciliated cells: 96 nCPM
Immune cell
- non-classical monocyte: 1.3 nTPM
- intermediate monocyte: 1 nTPM
- classical monocyte: 0.8 nTPM
- myeloid DC: 0.5 nTPM
- plasmacytoid DC: 0.4 nTPM
- NK-cell: 0.3 nTPM
Brain region
- cerebral cortex: 24 nTPM
- basal ganglia: 21 nTPM
- hippocampal formation: 20 nTPM
- amygdala: 19 nTPM
- hypothalamus: 19 nTPM
- pons: 19 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.83
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.86
- DepMap mean gene effect
- 0.05
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads DENND6B as an antibody target. Whether an autoantibody or antibody against DENND6B could matter depends on whether native DENND6B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
DENND6B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label DENND6B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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