Seroatlas · Human Serome Atlas

DENND2D

DENN domain-containing protein 2D

Also known as: DEN2D_HUMAN, FLJ22457, RP5-1180E21.2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H6A0
Gene: DENND2D
Ensembl: ENSG00000162777
Chromosome: 1
Canonical length: 471 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables guanyl-nucleotide exchange factor activity. Located in cytosol and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

471 residues, UniProt reviewed canonical sequence.

>Q9H6A0|DENND2D
     1  MEGQVVGRVF RLFQRRLLQL RAGPPQDNSG EALKEPERAQ EHSLPNFAGG QHFFEYLLVV
    61  SLKKKRSEDD YEPIITYQFP KRENLLRGQQ EEEERLLKAI PLFCFPDGNE WASLTEYPRE
   121  TFSFVLTNVD GSRKIGYCRR LLPAGPGPRL PKVYCIISCI GCFGLFSKIL DEVEKRHQIS
   181  MAVIYPFMQG LREAAFPAPG KTVTLKSFIP DSGTEFISLT RPLDSHLEHV DFSSLLHCLS
   241  FEQILQIFAS AVLERKIIFL AEGLSTLSQC IHAAAALLYP FSWAHTYIPV VPESLLATVC
   301  CPTPFMVGVQ MRFQQEVMDS PMEEVLLVNL CEGTFLMSVG DEKDILPPKL QDDILDSLGQ
   361  GINELKTAEQ INEHVSGPFV QFFVKIVGHY ASYIKREANG QGHFQERSFC KALTSKTNRR
   421  FVKKFVKTQL FSLFIQEAEK SKNPPAGYFQ QKILEYEEQK KQKKPREKTV K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DENND2D can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 88 nTPM

Expression across tissuesHPA

Tissue

lymph node: 88 nTPM
tonsil: 75 nTPM
thymus: 39 nTPM
spleen: 38 nTPM
appendix: 37 nTPM
parathyroid gland: 31 nTPM

Single-cell type

esophageal apical cells: 220 nCPM
nk-cells: 112 nCPM
hofbauer cells: 108 nCPM
esophageal suprabasal cells: 99 nCPM
late spermatids: 89 nCPM
late primary spermatocytes: 77 nCPM

Immune cell

NK-cell: 279 nTPM
naive CD4 T-cell: 266 nTPM
naive CD8 T-cell: 258 nTPM
memory CD8 T-cell: 244 nTPM
gdT-cell: 237 nTPM
MAIT T-cell: 205 nTPM

Brain region

choroid plexus: 7.7 nTPM
thalamus: 6.2 nTPM
white matter: 5.7 nTPM
medulla oblongata: 4 nTPM
spinal cord: 3.6 nTPM
hypothalamus: 2.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.64
gnomAD pLI: 0
gnomAD missense Z: 1.13
DepMap mean gene effect: -0.15
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

guanyl-nucleotide exchange factor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DENND2D as an antibody target. Whether an autoantibody or antibody against DENND2D could matter depends on whether native DENND2D is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DENND2D is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DENND2D as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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