Seroatlas · Human Serome Atlas

DDO

D-aspartate oxidase

Also known as: DASOX, DASPO, OXDD_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q99489
Gene: DDO
Ensembl: ENSG00000203797
Chromosome: 6
Canonical length: 341 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2019]

Canonical amino-acid sequenceUniProt

341 residues, UniProt reviewed canonical sequence.

>Q99489|DDO
     1  MDTARIAVVG AGVVGLSTAV CISKLVPRCS VTIISDKFTP DTTSDVAAGM LIPHTYPDTP
    61  IHTQKQWFRE TFNHLFAIAN SAEAGDAGVH LVSGWQIFQS TPTEEVPFWA DVVLGFRKMT
   121  EAELKKFPQY VFGQAFTTLK CECPAYLPWL EKRIKGSGGW TLTRRIEDLW ELHPSFDIVV
   181  NCSGLGSRQL AGDSKIFPVR GQVLQVQAPW VEHFIRDGSG LTYIYPGTSH VTLGGTRQKG
   241  DWNLSPDAEN SREILSRCCA LEPSLHGACN IREKVGLRPY RPGVRLQTEL LARDGQRLPV
   301  VHHYGHGSGG ISVHWGTALE AARLVSECVH ALRTPIPKSN L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDO can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 17 nTPM

Expression across tissuesHPA

Tissue

heart muscle: 17 nTPM
liver: 10 nTPM
skeletal muscle: 10 nTPM
kidney: 9.9 nTPM
basal ganglia: 8.6 nTPM
adrenal gland: 7.6 nTPM

Single-cell type

cardiomyocytes: 52 nCPM
myonuclei: 16 nCPM
paneth cells: 12 nCPM
oligodendrocytes: 10 nCPM
fallopian tube ciliated cells: 10 nCPM
müller glia: 9.4 nCPM

Immune cell

myeloid DC: 4.7 nTPM
intermediate monocyte: 3.3 nTPM
classical monocyte: 2.9 nTPM
NK-cell: 1.1 nTPM
total PBMC: 1.1 nTPM
non-classical monocyte: 1 nTPM

Brain region

basal ganglia: 10 nTPM
white matter: 7.5 nTPM
medulla oblongata: 6.9 nTPM
cerebellum: 6 nTPM
midbrain: 5.8 nTPM
pons: 5.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.24
gnomAD pLI: 0
gnomAD missense Z: -0.43
DepMap mean gene effect: -0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

aspartate metabolic process
D-amino acid catabolic process
grooming behavior
hormone metabolic process
insemination
L-aspartate catabolic process
nervous system process
L-amino acid catabolic process
proteinogenic amino acid catabolic process
regulation of cell communication

Molecular functions

FAD binding
D-aspartate oxidase activity
D-glutamate oxidase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DDO in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DDO as an antibody target. Whether an autoantibody or antibody against DDO could matter depends on whether native DDO is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DDO is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DDO as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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