DDAH1
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
Also known as: DDAH, DDAH1_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O94760
- Gene
- DDAH1
- Ensembl
- ENSG00000153904
- Chromosome
- 1
- Canonical length
- 285 aa
- Protein class
- Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
- Subcellular location
- Nucleoli,Plasma membrane,Cytosol
OverviewNCBI Gene
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
285 residues, UniProt reviewed canonical sequence.
>O94760|DDAH1
1 MAGLGHPAAF GRATHAVVRA LPESLGQHAL RSAKGEEVDV ARAERQHQLY VGVLGSKLGL
61 QVVELPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK EVDMMKEALE KLQLNIVEMK
121 DENATLDGGD VLFTGREFFV GLSKRTNQRG AEILADTFKD YAVSTVPVAD GLHLKSFCSM
181 AGPNLIAIGS SESAQKALKI MQQMSDHRYD KLTVPDDIAA NCIYLNIPNK GHVLLHRTPE
241 EYPESAKVYE KLKDHMLIPV SMSELEKVDG LLTCCSVLIN KKVDSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against DDAH1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.24
- Highest tissue expression
- 117 nTPM
Expression across tissuesHPA
Tissue
- kidney: 117 nTPM
- cerebral cortex: 105 nTPM
- basal ganglia: 98 nTPM
- amygdala: 95 nTPM
- liver: 74 nTPM
- epididymis: 72 nTPM
Single-cell type
- hepatic stellate cells: 426 nCPM
- astrocytes: 423 nCPM
- alveolar cells type 1: 338 nCPM
- ependymal cells: 331 nCPM
- proximal tubule cells: 291 nCPM
- distal convoluted tubule cells: 284 nCPM
Immune cell
- memory B-cell: 0.1 nTPM
- naive B-cell: 0.1 nTPM
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
Brain region
- thalamus: 185 nTPM
- cerebral cortex: 182 nTPM
- hippocampal formation: 174 nTPM
- basal ganglia: 171 nTPM
- amygdala: 162 nTPM
- midbrain: 140 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.95
- gnomAD pLI
- 0
- gnomAD missense Z
- 1.49
- DepMap mean gene effect
- 0.05
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- arginine metabolic process
- citrulline metabolic process
- L-arginine catabolic process
- negative regulation of cell population proliferation
- negative regulation of cellular response to hypoxia
- negative regulation of vascular permeability
- nitric oxide mediated signal transduction
- nitric oxide metabolic process
- positive regulation of angiogenesis
- positive regulation of nitric oxide biosynthetic process
- regulation of systemic arterial blood pressure
Molecular functions
- amino acid binding
- catalytic activity
- metal ion binding
- dimethylargininase activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Dimethylarginine dimethylaminohydrolase-like
- N,N dimethylarginine dimethylhydrolase, eukaryotic
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads DDAH1 as an antibody target. Whether an autoantibody or antibody against DDAH1 could matter depends on whether native DDAH1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
DDAH1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label DDAH1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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