Seroatlas · Human Serome Atlas

DBF4B

Protein DBF4 homolog B

Also known as: ASKL1, chifb, DBF4B_HUMAN, DRF1, FLJ13087, ZDBF1B

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NFT6
Gene: DBF4B
Ensembl: ENSG00000161692
Chromosome: 17
Canonical length: 615 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles

OverviewNCBI Gene

This gene encodes a regulator of the cell division cycle 7 homolog (S. cerevisiae) protein, a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and, in complex with the cell division cycle 7 homolog (S. cerevisiae) protein, may facilitate M phase progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Canonical amino-acid sequenceUniProt

615 residues, UniProt reviewed canonical sequence.

>Q8NFT6|DBF4B
     1  MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG KSFYLDLPAG
    61  KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS GKSHRGCPSP SPSEVRVETS
   121  AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ KAIRNQGSIS GGGSGGSSSL LTNARSWGVR
   181  ILHVDEMMMH VQQLSLASLC VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF
   241  RPFHHQFKSF PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY
   301  CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ AGLPRWSGSP
   361  ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG RAAGQQRWTE SLDGVMGPPA
   421  SHTCVSATTL LPALPKGSRE QGCLCPCPAS FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE
   481  NSFAPADIPV KGPLLFPEAR PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR
   541  LGYLYLLLTQ SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT
   601  PQPFLHCGFL AVDSG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DBF4B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.63
Highest tissue expression: 4.8 nTPM

Expression across tissuesHPA

Tissue

testis: 4.8 nTPM
epididymis: 3.3 nTPM
retina: 2.7 nTPM
thyroid gland: 2.6 nTPM
prostate: 2.2 nTPM
endometrium: 2.1 nTPM

Single-cell type

early primary spermatocytes: 91 nCPM
retinal amacrine cells: 45 nCPM
retinal bipolar cells: 41 nCPM
cardiomyocytes: 37 nCPM
undifferentiated spermatogonia: 37 nCPM
myonuclei: 36 nCPM

Immune cell

eosinophil: 0.4 nTPM
naive CD8 T-cell: 0.4 nTPM
intermediate monocyte: 0.3 nTPM
memory B-cell: 0.3 nTPM
non-classical monocyte: 0.3 nTPM
classical monocyte: 0.2 nTPM

Brain region

cerebral cortex: 2.9 nTPM
white matter: 2.3 nTPM
cerebellum: 2.2 nTPM
thalamus: 2.2 nTPM
basal ganglia: 2.1 nTPM
medulla oblongata: 2.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.17
gnomAD pLI: 0
gnomAD missense Z: 0.85
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DBF4B as an antibody target. Whether an autoantibody or antibody against DBF4B could matter depends on whether native DBF4B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DBF4B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DBF4B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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