CYP4X1

Protein identityUniProt · HPA

UniProt accession

Q8N118

Gene

CYP4X1

Ensembl

ENSG00000186377

Chromosome

1

Canonical length

509 aa

Protein class

Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the cytochrome P450 superfamily of enzymes and is located within a cluster of genes belonging to this superfamily on chromosome 1. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The expression pattern of a similar rat protein suggests that this protein may be involved in neurovascular function in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

509 residues, UniProt reviewed canonical sequence.

>Q8N118|CYP4X1
     1  MEFSWLETRW ARPFYLAFVF CLALGLLQAI KLYLRRQRLL RDLRPFPAPP THWFLGHQKF
    61  IQDDNMEKLE EIIEKYPRAF PFWIGPFQAF FCIYDPDYAK TLLSRTDPKS QYLQKFSPPL
   121  LGKGLAALDG PKWFQHRRLL TPGFHFNILK AYIEVMAHSV KMMLDKWEKI CSTQDTSVEV
   181  YEHINSMSLD IIMKCAFSKE TNCQTNSTHD PYAKAIFELS KIIFHRLYSL LYHSDIIFKL
   241  SPQGYRFQKL SRVLNQYTDT IIQERKKSLQ AGVKQDNTPK RKYQDFLDIV LSAKDESGSS
   301  FSDIDVHSEV STFLLAGHDT LAASISWILY CLALNPEHQE RCREEVRGIL GDGSSITWDQ
   361  LGEMSYTTMC IKETCRLIPA VPSISRDLSK PLTFPDGCTL PAGITVVLSI WGLHHNPAVW
   421  KNPKVFDPLR FSQENSDQRH PYAYLPFSAG SRNCIGQEFA MIELKVTIAL ILLHFRVTPD
   481  PTRPLTFPNH FILKPKNGMY LHLKKLSEC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYP4X1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

35 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.11

gnomAD pLI

0

gnomAD missense Z

1.29

DepMap mean gene effect

-0.12

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• lipid metabolic process

Molecular functions

• anandamide 14,15 epoxidase activity
• heme binding
• iron ion binding

Cellular components

• endoplasmic reticulum membrane

Protein domainsUniProt · Pfam · InterPro

• Cytochrome P450
• Cytochrome P450, E-class, group I
• Cytochrome P450, conserved site
• Cytochrome P450 superfamily
• Cytochrome P450 Monooxygenases
• Cytochrome P450

KeywordsUniProt

• Endoplasmic reticulum
• Heme
• Iron
• Lipid metabolism
• Membrane
• Metal-binding
• Microsome
• Monooxygenase
• Oxidoreductase
• Transmembrane
• Transmembrane helix