CYP4F12

Protein identityUniProt · HPA

UniProt accession

Q9HCS2

Gene

CYP4F12

Ensembl

ENSG00000186204

Chromosome

19

Canonical length

524 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum. When expressed in yeast the enzyme is capable of oxdizing arachidonic acid. It can also catalyze the epoxidation of 22:6n-3 and 22:5n-3 polyunsaturated long-chain fatty acids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Canonical amino-acid sequenceUniProt

524 residues, UniProt reviewed canonical sequence.

>Q9HCS2|CYP4F12
     1  MSLLSLPWLG LRPVATSPWL LLLLVVGSWL LARILAWTYA FYNNCRRLQC FPQPPKRNWF
    61  WGHLGLITPT EEGLKNSTQM SATYSQGFTV WLGPIIPFIV LCHPDTIRSI TNASAAIAPK
   121  DNLFIRFLKP WLGEGILLSG GDKWSRHRRM LTPAFHFNIL KSYITIFNKS ANIMLDKWQH
   181  LASEGSSRLD MFEHISLMTL DSLQKCIFSF DSHCQERPSE YIATILELSA LVEKRSQHIL
   241  QHMDFLYYLS HDGRRFHRAC RLVHDFTDAV IRERRRTLPT QGIDDFFKDK AKSKTLDFID
   301  VLLLSKDEDG KALSDEDIRA EADTFMFGGH DTTASGLSWV LYNLARHPEY QERCRQEVQE
   361  LLKDRDPKEI EWDDLAQLPF LTMCVKESLR LHPPAPFISR CCTQDIVLPD GRVIPKGITC
   421  LIDIIGVHHN PTVWPDPEVY DPFRFDPENS KGRSPLAFIP FSAGPRNCIG QAFAMAEMKV
   481  VLALMLLHFR FLPDHTEPRR KLELIMRAEG GLWLRVEPLN VSLQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYP4F12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

2

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

41 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.38

gnomAD pLI

0

gnomAD missense Z

-0.76

DepMap mean gene effect

-0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• arachidonate metabolic process
• epoxygenase P450 pathway
• fatty acid metabolic process
• leukotriene B4 catabolic process
• long-chain fatty acid metabolic process
• menaquinone catabolic process
• phylloquinone catabolic process
• pressure natriuresis
• renal water homeostasis
• sodium ion homeostasis
• very long-chain fatty acid metabolic process
• vitamin E metabolic process
• xenobiotic metabolic process

Molecular functions

• alkane 1-monooxygenase activity
• arachidonate epoxygenase activity
• heme binding
• iron ion binding
• leukotriene-B4 20-monooxygenase activity
• monooxygenase activity

Cellular components

• apical plasma membrane
• cytoplasm
• endoplasmic reticulum membrane
• intracellular membrane-bounded organelle

Protein domainsUniProt · Pfam · InterPro

• Cytochrome P450
• Cytochrome P450, E-class, group I
• Cytochrome P450, conserved site
• Cytochrome P450 superfamily
• Cytochrome P450 Monooxygenases
• Cytochrome P450

KeywordsUniProt

• Endoplasmic reticulum
• Fatty acid metabolism
• Heme
• Iron
• Lipid metabolism
• Membrane
• Metal-binding
• Microsome
• Monooxygenase
• Oxidoreductase
• Transmembrane
• Transmembrane helix