Seroatlas · Human Serome Atlas

CYB561D2

Transmembrane reductase CYB561D2

Also known as: 101F6, C56D2_HUMAN, TSP10

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O14569
Gene: CYB561D2
Ensembl: ENSG00000114395
Chromosome: 3
Canonical length: 222 aa
Protein class: Enzymes, Predicted membrane proteins

OverviewNCBI Gene

Enables heme binding activity and transmembrane monodehydroascorbate reductase activity. Involved in ascorbate homeostasis. Predicted to be located in vesicle. Predicted to be active in endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

222 residues, UniProt reviewed canonical sequence.

>O14569|CYB561D2
     1  MALSAETESH IYRALRTASG AAAHLVALGF TIFVAVLARP GSSLFSWHPV LMSLAFSFLM
    61  TEALLVFSPE SSLLHSLSRK GRARCHWVLQ LLALLCALLG LGLVILHKEQ LGKAHLVTRH
   121  GQAGLLAVLW AGLQCSGGVG LLYPKLLPRW PLAKLKLYHA TSGLVGYLLG SASLLLGMCS
   181  LWFTASVTGA AWYLAVLCPV LTSLVIMNQV SNAYLYRKRI QP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYB561D2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 6
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 36 nTPM

Expression across tissuesHPA

Tissue

liver: 36 nTPM
choroid plexus: 26 nTPM
spleen: 25 nTPM
epididymis: 24 nTPM
small intestine: 23 nTPM
stomach: 22 nTPM

Single-cell type

melanocytes: 2 nCPM
gastric chief cells: 1.1 nCPM
cardiomyocytes: 0.7 nCPM
breast lactating cells: 0.6 nCPM
enterocytes: 0.5 nCPM
hematopoietic stem cells: 0.5 nCPM

Immune cell

eosinophil: 177 nTPM
basophil: 146 nTPM
gdT-cell: 145 nTPM
intermediate monocyte: 140 nTPM
non-classical monocyte: 137 nTPM
memory CD8 T-cell: 116 nTPM

Brain region

white matter: 16 nTPM
choroid plexus: 14 nTPM
medulla oblongata: 13 nTPM
spinal cord: 12 nTPM
basal ganglia: 12 nTPM
cerebellum: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.34
gnomAD pLI: 0
gnomAD missense Z: 0.97
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

ascorbate homeostasis

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CYB561D2 as an antibody target. Whether an autoantibody or antibody against CYB561D2 could matter depends on whether native CYB561D2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CYB561D2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CYB561D2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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