Seroatlas · Human Serome Atlas

CYB561D1

Probable transmembrane reductase CYB561D1

Also known as: C56D1_HUMAN, FLJ39035, FLJ44753

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N8Q1
Gene: CYB561D1
Ensembl: ENSG00000174151
Chromosome: 1
Canonical length: 229 aa
Protein class: Enzymes, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

Predicted to enable metal ion binding activity; transmembrane ascorbate ferrireductase activity; and transmembrane monodehydroascorbate reductase activity. Predicted to be involved in transmembrane transport. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

229 residues, UniProt reviewed canonical sequence.

>Q8N8Q1|CYB561D1
     1  MQPLEVGLVP APAGEPRLTR WLRRGSGILA HLVALGFTIF LTALSRPGTS LFSWHPVFMA
    61  LAFCLCMAEA ILLFSPEHSL FFFCSRKARI RLHWAGQTLA ILCAALGLGF IISSRTRSEL
   121  PHLVSWHSWV GALTLLATAV QALCGLCLLC PRAARVSRVA RLKLYHLTCG LVVYLMATVT
   181  VLLGMYSVWF QAQIKGAAWY LCLALPVYPA LVIMHQISRS YLPRKKMEM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYB561D1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 6
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 17 nTPM

Expression across tissuesHPA

Tissue

skin: 17 nTPM
cerebellum: 14 nTPM
pancreas: 11 nTPM
epididymis: 10 nTPM
lymph node: 10 nTPM
tonsil: 9.6 nTPM

Single-cell type

gonadotrophs: 23 nCPM
enterocytes: 17 nCPM
lactotrophs: 16 nCPM
microglia: 16 nCPM
brain excitatory neurons: 15 nCPM
retinal bipolar cells: 15 nCPM

Immune cell

neutrophil: 6.7 nTPM
NK-cell: 5.6 nTPM
naive CD4 T-cell: 4.9 nTPM
memory CD4 T-cell: 3.9 nTPM
gdT-cell: 3.7 nTPM
eosinophil: 3.6 nTPM

Brain region

cerebellum: 16 nTPM
hippocampal formation: 15 nTPM
cerebral cortex: 14 nTPM
amygdala: 13 nTPM
basal ganglia: 12 nTPM
thalamus: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.5
gnomAD pLI: 0
gnomAD missense Z: -0.07
DepMap mean gene effect: -0.21
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CYB561D1 as an antibody target. Whether an autoantibody or antibody against CYB561D1 could matter depends on whether native CYB561D1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CYB561D1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CYB561D1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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