Seroatlas · Human Serome Atlas

CXorf65

Uncharacterized protein CXorf65

Also known as: CX065_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A6NEN9
Gene: CXorf65
Ensembl: ENSG00000204165
Chromosome: X
Canonical length: 183 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to act upstream of or within transcription by RNA polymerase II. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

183 residues, UniProt reviewed canonical sequence.

>A6NEN9|CXorf65
     1  MFIFIKHGDN QQFLVNTNCA VVVLLYYIRS KVKLPKTNTI DLCEQTGKMK MLFLMKPNHA
    61  EYASKYLTAR STYYVCKVER GPPGTRLENA YRAFVPLLKN PEPWLLVALR IQCDALERRR
   121  IQMLKMKEAK KVVIIEPPAS VPSKQSGRSD KKKSTRKSPT FRNRPDFRKN KGRQLNKTTK
   181  QKK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CXorf65 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 25 nTPM

Expression across tissuesHPA

Tissue

testis: 25 nTPM
bone marrow: 0.6 nTPM
spleen: 0.5 nTPM
small intestine: 0.4 nTPM
tonsil: 0.3 nTPM
appendix: 0.2 nTPM

Single-cell type

late spermatids: 2 nCPM
early spermatids: 0.4 nCPM
müller glia: 0.3 nCPM
enterocytes: 0.1 nCPM
adipocytes: 0 nCPM
adrenal cortex cells: 0 nCPM

Immune cell

NK-cell: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

medulla oblongata: 0.1 nTPM
white matter: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM
cerebellum: 0 nTPM
cerebral cortex: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.43
gnomAD pLI: 0.88
gnomAD missense Z: 0.2
DepMap mean gene effect: 0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

transcription by RNA polymerase II

Molecular functions

RNA polymerase II cis-regulatory region sequence-specific DNA binding

Protein domainsUniProt · Pfam · InterPro

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CXorf65 as an antibody target. Whether an autoantibody or antibody against CXorf65 could matter depends on whether native CXorf65 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CXorf65 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CXorf65 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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