Seroatlas · Human Serome Atlas

CWC25

Pre-mRNA-splicing factor CWC25 homolog

Also known as: CCDC49, CWC25_HUMAN, FLJ20291

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NXE8
Gene: CWC25
Ensembl: ENSG00000273559
Chromosome: 17
Canonical length: 425 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nuclear speckles

OverviewNCBI Gene

This gene encodes a factor that is part of the multi-protein C complex involved in pre-mRNA splicing. Alternatively spliced transcripts have been described for this gene. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

425 residues, UniProt reviewed canonical sequence.

>Q9NXE8|CWC25
     1  MGGGDLNLKK SWHPQTLRNV EKVWKAEQKH EAERKKIEEL QRELREERAR EEMQRYAEDV
    61  GAVKKKEEKL DWMYQGPGGM VNRDEYLLGR PIDKYVFEKM EEKEAGCSSE TGLLPGSIFA
   121  PSGANSLLDM ASKIREDPLF IIRKKEEEKK REVLNNPVKM KKIKELLQMS LEKKEKKKKK
   181  EKKKKHKKHK HRSSSSDRSS SEDEHSAGRS QKKMANSSPV LSKVPGYGLQ VRNSDRNQGL
   241  QGPLTAEQKR GHGMKNHSRS RSSSHSPPRH ASKKSTREAG SRDRRSRSLG RRSRSPRPSK
   301  LHNSKVNRRE TGQTRSPSPK KEVYQRRHAP GYTRKLSAEE LERKRQEMME NAKWREEERL
   361  NILKRHAKDE EREQRLEKLD SRDGKFIHRM KLESASTSSL EDRVKRNIYS LQRTSVALEK
   421  NFMKR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CWC25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.6
Highest tissue expression: 59 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 59 nTPM
testis: 19 nTPM
skin: 18 nTPM
spleen: 17 nTPM
liver: 17 nTPM
choroid plexus: 14 nTPM

Single-cell type

early spermatids: 78 nCPM
oocytes: 74 nCPM
late primary spermatocytes: 59 nCPM
syncytiotrophoblasts: 48 nCPM
enterocytes: 46 nCPM
neutrophils: 44 nCPM

Immune cell

neutrophil: 28 nTPM
basophil: 18 nTPM
eosinophil: 10 nTPM
non-classical monocyte: 8.1 nTPM
naive B-cell: 5.9 nTPM
NK-cell: 5.8 nTPM

Brain region

white matter: 9.3 nTPM
choroid plexus: 8.3 nTPM
basal ganglia: 8.2 nTPM
cerebral cortex: 8.1 nTPM
medulla oblongata: 8.1 nTPM
pons: 7.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.79
gnomAD pLI: 0
gnomAD missense Z: 0.65
DepMap mean gene effect: -0.37
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

via spliceosome
mRNA splicing

Cellular components

Protein domainsUniProt · Pfam · InterPro

CBF1-interacting co-repressor CIR, N-terminal domain
N-terminal domain of CBF1 interacting co-repressor CIR
Pre-mRNA splicing factor
Pre-mRNA-splicing factor CWC25
Pre-mRNA splicing factor

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CWC25 as an antibody target. Whether an autoantibody or antibody against CWC25 could matter depends on whether native CWC25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CWC25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CWC25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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