CTSH

Protein identityUniProt · HPA

UniProt accession

P09668

Gene

CTSH

Ensembl

ENSG00000103811

Chromosome

15

Canonical length

335 aa

Protein class

Cancer-related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Vesicles,Cytosol,Cytoplasmic bodies

Secretome location

Intracellular and membrane

OverviewNCBI Gene

The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Canonical amino-acid sequenceUniProt

335 residues, UniProt reviewed canonical sequence.

>P09668|CTSH
     1  MWATLPLLCA GAWLLGVPVC GAAELCVNSL EKFHFKSWMS KHRKTYSTEE YHHRLQTFAS
    61  NWRKINAHNN GNHTFKMALN QFSDMSFAEI KHKYLWSEPQ NCSATKSNYL RGTGPYPPSV
   121  DWRKKGNFVS PVKNQGACGS CWTFSTTGAL ESAIAIATGK MLSLAEQQLV DCAQDFNNHG
   181  CQGGLPSQAF EYILYNKGIM GEDTYPYQGK DGYCKFQPGK AIGFVKDVAN ITIYDEEAMV
   241  EAVALYNPVS FAFEVTQDFM MYRTGIYSST SCHKTPDKVN HAVLAVGYGE KNGIPYWIVK
   301  NSWGPQWGMN GYFLIERGKN MCGLAACASY PIPLV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CTSH can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

330 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CTSH.

Disease | ImmuneIEDB

Conditions an epitope on CTSH was assayed in.

• chronic lymphocytic leukemia T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.01

gnomAD pLI

0

gnomAD missense Z

0.54

DepMap mean gene effect

0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• adaptive immune response
• antigen processing and presentation
• bradykinin catabolic process
• cellular response to thyroid hormone stimulus
• ERK1 and ERK2 cascade
• immune response
• immune response-regulating signaling pathway
• lysosomal protein catabolic process
• membrane protein proteolysis
• metanephros development
• neuropeptide catabolic process
• positive regulation of apoptotic signaling pathway
• positive regulation of cell migration
• positive regulation of epithelial cell migration
• positive regulation of gene expression
• protein destabilization
• proteolysis
• proteolysis involved in protein catabolic process
• response to odorant
• response to retinoic acid
• spermatogenesis
• surfactant homeostasis
• T cell mediated cytotoxicity
• zymogen activation
• dichotomous subdivision of terminal units involved in lung branching

Molecular functions

• aminopeptidase activity
• cysteine-type endopeptidase activator activity involved in apoptotic process
• cysteine-type endopeptidase activity
• cysteine-type peptidase activity
• endopeptidase activity
• HLA-A specific activating MHC class I receptor activity
• identical protein binding
• kininogen binding
• peptidase activity
• protein-containing complex binding
• serine-type endopeptidase activity
• thyroid hormone binding

Cellular components

• acrosomal vesicle
• alveolar lamellar body
• axoneme
• cytoplasmic ribonucleoprotein granule
• cytosol
• extracellular exosome
• extracellular matrix
• extracellular region
• extracellular space
• ficolin-1-rich granule lumen
• lysosome
• multivesicular body lumen
• outer dense fiber
• secretory granule lumen
• tertiary granule lumen

Protein domainsUniProt · Pfam · InterPro

• Cysteine peptidase, cysteine active site
• Peptidase C1A, papain C-terminal
• Peptidase C1A
• Cathepsin propeptide inhibitor domain (I29)
• Cysteine peptidase, histidine active site
• Cysteine peptidase, asparagine active site
• Papain-like cysteine peptidase superfamily
• Papain-like cysteine endopeptidase
• Papain family cysteine protease
• Cathepsin propeptide inhibitor domain (I29)

KeywordsUniProt

• Disulfide bond
• Glycoprotein
• Hydrolase
• Lysosome
• Protease
• Signal
• Thiol protease
• Zymogen

InteractionsUniProt · HPA

Protein binding partners of CTSH in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CSTA