Seroatlas · Human Serome Atlas

CMBL

Carboxymethylenebutenolidase homolog

Also known as: CMBL_HUMAN, FLJ23617

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96DG6
Gene: CMBL
Ensembl: ENSG00000164237
Chromosome: 5
Canonical length: 245 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010]

Canonical amino-acid sequenceUniProt

245 residues, UniProt reviewed canonical sequence.

>Q96DG6|CMBL
     1  MANEAYPCPC DIGHRLEYGG LGREVQVEHI KAYVTKSPVD AGKAVIVIQD IFGWQLPNTR
    61  YIADMISGNG YTTIVPDFFV GQEPWDPSGD WSIFPEWLKT RNAQKIDREI SAILKYLKQQ
   121  CHAQKIGIVG FCWGGTAVHH LMMKYSEFRA GVSVYGIVKD SEDIYNLKNP TLFIFAENDV
   181  VIPLKDVSLL TQKLKEHCKV EYQIKTFSGQ THGFVHRKRE DCSPADKPYI DEARRNLIEW
   241  LNKYM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CMBL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 161 nTPM

Expression across tissuesHPA

Tissue

liver: 161 nTPM
kidney: 157 nTPM
skeletal muscle: 154 nTPM
duodenum: 90 nTPM
small intestine: 80 nTPM
tongue: 40 nTPM

Single-cell type

enterocytes: 717 nCPM
hepatocytes: 232 nCPM
enteric transient amplifying cells: 214 nCPM
colonocytes: 205 nCPM
enteric stem cells: 153 nCPM
thymic myoid cells: 136 nCPM

Immune cell

basophil: 0.2 nTPM
MAIT T-cell: 0.2 nTPM
neutrophil: 0.2 nTPM
classical monocyte: 0.1 nTPM
gdT-cell: 0.1 nTPM
memory CD4 T-cell: 0.1 nTPM

Brain region

hypothalamus: 14 nTPM
thalamus: 12 nTPM
midbrain: 12 nTPM
pons: 12 nTPM
cerebral cortex: 12 nTPM
amygdala: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.11
gnomAD pLI: 0
gnomAD missense Z: -0.37
DepMap mean gene effect: 0.16
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

xenobiotic metabolic process

Molecular functions

hydrolase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

Alpha/Beta hydrolase fold
Dienelactone hydrolase
Carboxymethylenebutenolidase homolog
Dienelactone hydrolase family

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CMBL as an antibody target. Whether an autoantibody or antibody against CMBL could matter depends on whether native CMBL is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CMBL is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CMBL as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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