Seroatlas · Human Serome Atlas

CLYBL

Citramalyl-CoA lyase, mitochondrial

Also known as: CLB, CLYBL_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N0X4
Gene: CLYBL
Ensembl: ENSG00000125246
Chromosome: 13
Canonical length: 340 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins
Quaternary structure: Homotrimer

OverviewNCBI Gene

Enables (S)-citramalyl-CoA lyase activity; magnesium ion binding activity; and malate synthase activity. Involved in protein homotrimerization and regulation of cobalamin metabolic process. Located in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

340 residues, UniProt reviewed canonical sequence.

>Q8N0X4|CLYBL
     1  MALRLLRRAA RGAAAAALLR LKASLAADIP RLGYSSSSHH KYIPRRAVLY VPGNDEKKIK
    61  KIPSLNVDCA VLDCEDGVAA NKKNEARLRI VKTLEDIDLG PTEKCVRVNS VSSGLAEEDL
   121  ETLLQSRVLP SSLMLPKVES PEEIQWFADK FSFHLKGRKL EQPMNLIPFV ETAMGLLNFK
   181  AVCEETLKVG PQVGLFLDAV VFGGEDFRAS IGATSSKETL DILYARQKIV VIAKAFGLQA
   241  IDLVYIDFRD GAGLLRQSRE GAAMGFTGKQ VIHPNQIAVV QEQFSPSPEK IKWAEELIAA
   301  FKEHQQLGKG AFTFQGSMID MPLLKQAQNT VTLATSIKEK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CLYBL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 100 nTPM

Expression across tissuesHPA

Tissue

liver: 100 nTPM
choroid plexus: 65 nTPM
tongue: 64 nTPM
kidney: 59 nTPM
skeletal muscle: 40 nTPM
duodenum: 23 nTPM

Single-cell type

choroid plexus epithelial cells: 518 nCPM
renal collecting duct intercalated cells: 326 nCPM
ependymal cells: 267 nCPM
bergmann glia: 257 nCPM
proximal tubule cells: 216 nCPM
hepatocytes: 202 nCPM

Immune cell

T-reg: 24 nTPM
memory CD4 T-cell: 17 nTPM
naive CD8 T-cell: 16 nTPM
naive B-cell: 15 nTPM
memory B-cell: 14 nTPM
NK-cell: 14 nTPM

Brain region

choroid plexus: 70 nTPM
cerebellum: 46 nTPM
cerebral cortex: 26 nTPM
hippocampal formation: 26 nTPM
basal ganglia: 24 nTPM
amygdala: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.31
gnomAD pLI: 0
gnomAD missense Z: -0.08
DepMap mean gene effect: 0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein homotrimerization
positive regulation of cobalamin metabolic process
regulation of cobalamin metabolic process

Molecular functions

hydrolase activity
magnesium ion binding
(S)-citramalyl-CoA lyase activity
malate synthase activity

Cellular components

mitochondrion

Protein domainsUniProt · Pfam · InterPro

Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily
Pyruvate kinase-like domain superfamily
HpcH/HpaI aldolase/citrate lyase domain
Citrate lyase beta subunit-like
Citramalyl-CoA lyase
HpcH/HpaI aldolase/citrate lyase

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CLYBL as an antibody target. Whether an autoantibody or antibody against CLYBL could matter depends on whether native CLYBL is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CLYBL is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CLYBL as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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