Seroatlas · Human Serome Atlas

CKLF

Chemokine-like factor

Also known as: C32, CKLF_HUMAN, CKLF1, CKLF3, CKLF4, HSPC224, UCK-1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UBR5
Gene: CKLF
Ensembl: ENSG00000217555
Chromosome: 16
Canonical length: 152 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Predicted secreted proteins
Subcellular location: Vesicles,Mid piece,Principal piece
Secretome location: Secreted to blood

OverviewNCBI Gene

The product of this gene is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. The protein encoded by this gene is a potent chemoattractant for neutrophils, monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. This protein may play important roles in inflammation and in the regeneration of skeletal muscle. Alternatively spliced transcript variants encoding different isoforms have been identified. Naturally occurring read-through transcription occurs between this locus and the neighboring locus CMTM1 (CKLF-like MARVEL transmembrane domain containing 1).[provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

152 residues, UniProt reviewed canonical sequence.

>Q9UBR5|CKLF
     1  MDNVQPKIKH RPFCFSVKGH VKMLRLALTV TSMTFFIIAQ APEPYIVITG FEVTVILFFI
    61  LLYVLRLDRL MKWLFWPLLD IINSLVTTVF MLIVSVLALI PETTTLTVGG GVFALVTAVC
   121  CLADGALIYR KLLFNPSGPY QKKPVHEKKE VL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CKLF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 3
Mean surface accessibility (rSASA): 0.39
Highest tissue expression: 84 nTPM

Expression across tissuesHPA

Tissue

thymus: 84 nTPM
spleen: 65 nTPM
bone marrow: 64 nTPM
lymph node: 58 nTPM
parathyroid gland: 55 nTPM
testis: 54 nTPM

Single-cell type

gastric progenitor cells: 440 nCPM
neutrophils: 399 nCPM
late primary spermatocytes: 361 nCPM
mast cells: 259 nCPM
megakaryocytes: 210 nCPM
hofbauer cells: 206 nCPM

Immune cell

neutrophil: 1,394 nTPM
basophil: 811 nTPM
eosinophil: 776 nTPM
total PBMC: 402 nTPM
classical monocyte: 383 nTPM
myeloid DC: 319 nTPM

Brain region

white matter: 18 nTPM
hypothalamus: 16 nTPM
medulla oblongata: 16 nTPM
spinal cord: 14 nTPM
pons: 13 nTPM
cerebellum: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.51
gnomAD pLI: 0
gnomAD missense Z: -0.12
DepMap mean gene effect: -0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

chemokine activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CKLF as an antibody target. Whether an autoantibody or antibody against CKLF could matter depends on whether native CKLF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CKLF is annotated as secreted, so native CKLF circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label CKLF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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