Seroatlas · Human Serome Atlas

CHST8

Carbohydrate sulfotransferase 8

Also known as: CHST8_HUMAN, GALNAC4ST-1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H2A9
Gene
CHST8
Ensembl
ENSG00000124302
Chromosome
19
Canonical length
424 aa
Protein class
Disease related genes, Human disease related genes, Metabolic proteins, Predicted intracellular proteins
Secretome location
Intracellular and membrane

OverviewNCBI Gene

The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

424 residues, UniProt reviewed canonical sequence.

>Q9H2A9|CHST8
     1  MTLRPGTMRL ACMFSSILLF GAAGLLLFIS LQDPTELAPQ QVPGIKFNIR PRQPHHDLPP
    61  GGSQDGDLKE PTERVTRDLS SGAPRGRNLP APDQPQPPLQ RGTRLRLRQR RRRLLIKKMP
   121  AAATIPANSS DAPFIRPGPG TLDGRWVSLH RSQQERKRVM QEACAKYRAS SSRRAVTPRH
   181  VSRIFVEDRH RVLYCEVPKA GCSNWKRVLM VLAGLASSTA DIQHNTVHYG SALKRLDTFD
   241  RQGILHRLST YTKMLFVREP FERLVSAFRD KFEHPNSYYH PVFGKAILAR YRANASREAL
   301  RTGSGVRFPE FVQYLLDVHR PVGMDIHWDH VSRLCSPCLI DYDFVGKFES MEDDANFFLS
   361  LIRAPRNLTF PRFKDRHSQE ARTTARIAHQ YFAQLSALQR QRTYDFYYMD YLMFNYSKPF
   421  ADLY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHST8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
61 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 61 nTPM
  • cerebellum: 17 nTPM
  • hippocampal formation: 16 nTPM
  • spinal cord: 14 nTPM
  • hypothalamus: 13 nTPM
  • cerebral cortex: 11 nTPM

Single-cell type

  • lactotrophs: 473 nCPM
  • somatotrophs: 341 nCPM
  • gonadotrophs: 218 nCPM
  • corticotrophs: 158 nCPM
  • late spermatids: 133 nCPM
  • thyrotrophs: 127 nCPM

Immune cell

  • total PBMC: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM

Brain region

  • pons: 65 nTPM
  • medulla oblongata: 34 nTPM
  • hypothalamus: 26 nTPM
  • thalamus: 26 nTPM
  • cerebellum: 26 nTPM
  • hippocampal formation: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.12
gnomAD pLI
0
gnomAD missense Z
1.01
DepMap mean gene effect
-0.15
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHST8 as an antibody target. Whether an autoantibody or antibody against CHST8 could matter depends on whether native CHST8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHST8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHST8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CHST8. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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