Seroatlas · Human Serome Atlas

CHKA

Choline kinase alpha

Also known as: CHK, CHKA_HUMAN, CKI

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P35790
Gene: CHKA
Ensembl: ENSG00000110721
Chromosome: 11
Canonical length: 457 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Endoplasmic reticulum
Quaternary structure: Homodimer

OverviewNCBI Gene

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

457 residues, UniProt reviewed canonical sequence.

>P35790|CHKA
     1  MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG QQPPLALPPP
    61  PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP GAWRGLREDE FHISVIRGGL
   121  SNMLFQCSLP DTTATLGDEP RKVLLRLYGA ILQMRSCNKE GSEQAQKENE FQGAEAMVLE
   181  SVMFAILAER SLGPKLYGIF PQGRLEQFIP SRRLDTEELS LPDISAEIAE KMATFHGMKM
   241  PFNKEPKWLF GTMEKYLKEV LRIKFTEESR IKKLHKLLSY NLPLELENLR SLLESTPSPV
   301  VFCHNDCQEG NILLLEGREN SEKQKLMLID FEYSSYNYRG FDIGNHFCEW MYDYSYEKYP
   361  FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI IKEEMLLEVN RFALASHFLW
   421  GLWSIVQAKI SSIEFGYMDY AQARFDAYFH QKRKLGV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHKA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 71 nTPM

Expression across tissuesHPA

Tissue

testis: 71 nTPM
spinal cord: 43 nTPM
salivary gland: 40 nTPM
liver: 39 nTPM
midbrain: 39 nTPM
thyroid gland: 38 nTPM

Single-cell type

salivary duct cells: 451 nCPM
early spermatids: 388 nCPM
late primary spermatocytes: 362 nCPM
late spermatids: 361 nCPM
salivary acinar cells: 343 nCPM
neutrophil progenitors: 329 nCPM

Immune cell

eosinophil: 6.1 nTPM
intermediate monocyte: 1.5 nTPM
NK-cell: 1.1 nTPM
myeloid DC: 0.9 nTPM
non-classical monocyte: 0.9 nTPM
classical monocyte: 0.8 nTPM

Brain region

white matter: 80 nTPM
pons: 61 nTPM
medulla oblongata: 51 nTPM
thalamus: 46 nTPM
basal ganglia: 46 nTPM
choroid plexus: 45 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CHKA.

Disease | AllUniProt

Conditions CHKA is implicated in, by any mechanism.

Neurodevelopmental disorder with microcephaly, movement abnormalities, and seizures (NEDMIMS) MIM:620023

Disease | GeneticClinVar

5 pathogenic / likely-pathogenic of 76 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Microcephaly
Severe intellectual disability
Seizure
Neurodevelopmental disorder with microcephaly, movement abnormalities, and seizures

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.48
gnomAD pLI: 0.23
gnomAD missense Z: 1
DepMap mean gene effect: -0.37
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHKA as an antibody target. Whether an autoantibody or antibody against CHKA could matter depends on whether native CHKA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHKA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHKA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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