CD34

Protein identityUniProt · HPA

UniProt accession

P28906

Gene

CD34

Ensembl

ENSG00000174059

Chromosome

1

Canonical length

385 aa

Protein class

CD markers, Predicted membrane proteins

Subcellular location

Nucleoplasm,Plasma membrane,Cell Junctions

OverviewNCBI Gene

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

385 residues, UniProt reviewed canonical sequence.

>P28906|CD34
     1  MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS NVSTNVSYQE
    61  TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV
   121  FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI
   181  REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP
   241  QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL
   301  LAVLGITGYF LMNRRSWSPT GERLGEDPYY TENGGGQGYS SGPGTSPEAQ GKASVNRGAQ
   361  ENGTGQATSR NGHSARQHVV ADTEL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CD34 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.59

Highest tissue expression

206 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for CD34 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.02

gnomAD pLI

0

gnomAD missense Z

0.77

DepMap mean gene effect

0.11

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell motility
• cell-cell adhesion
• cell-matrix adhesion
• endothelial cell proliferation
• endothelium development
• glomerular endothelium development
• glomerular filtration
• hematopoietic stem cell proliferation
• hemopoiesis
• leukocyte migration
• mesangial cell-matrix adhesion
• negative regulation of gene expression
• negative regulation of interleukin-2 production
• paracrine signaling
• positive regulation of angiogenesis
• positive regulation of gene expression
• positive regulation of granulocyte colony-stimulating factor production
• positive regulation of odontogenesis
• positive regulation of vasculogenesis
• signal transduction
• stem cell proliferation
• tissue homeostasis
• transdifferentiation
• vascular wound healing
• extracellular exosome assembly
• metanephric glomerular mesangial cell differentiation

Molecular functions

• carbohydrate binding
• RNA polymerase II-specific DNA-binding transcription factor binding
• sulfate binding

Cellular components

• apical plasma membrane
• basal plasma membrane
• cytoplasm
• external side of plasma membrane
• extracellular region
• intercellular bridge
• lysosome
• perinuclear region of cytoplasm
• plasma membrane
• glomerular endothelium fenestra

Protein domainsUniProt · Pfam · InterPro

• CD34/Podocalyxin
• CD34/Podocalyxin family
• CD34 antigen

KeywordsUniProt

• Cell adhesion
• Glycoprotein
• Membrane
• Phosphoprotein
• Signal
• Transmembrane
• Transmembrane helix