Seroatlas · Human Serome Atlas

CASS4

Cas scaffolding protein family member 4

Also known as: C20orf32, CASS4_HUMAN, HEFL, HEPL

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NQ75
Gene
CASS4
Ensembl
ENSG00000087589
Chromosome
20
Canonical length
786 aa
Protein class
Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Enables protein tyrosine kinase binding activity. Involved in several processes, including positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; positive regulation of protein tyrosine kinase activity; and positive regulation of substrate adhesion-dependent cell spreading. Located in cytoplasm and focal adhesion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

786 residues, UniProt reviewed canonical sequence.

>Q9NQ75|CASS4
     1  MKGTGIMDCA PKALLARALY DNCPDCSDEL AFSRGDILTI LEQHVPESEG WWKCLLHGRQ
    61  GLAPANRLQI LTEVAADRPC PPFLRGLEEA PASSEETYQV PTLPRPPTPG PVYEQMRSWA
   121  EGPQPPTAQV YEFPDPPTSA RIICEKTLSF PKQAILTLPR PVRASLPTLP SQVYDVPTQH
   181  RGPVVLKEPE KQQLYDIPAS PKKAGLHPPD SQASGQGVPL ISVTTLRRGG YSTLPNPQKS
   241  EWIYDTPVSP GKASVRNTPL TSFAEESRPH ALPSSSSTFY NPPSGRSRSL TPQLNNNVPM
   301  QKKLSLPEIP SYGFLVPRGT FPLDEDVSYK VPSSFLIPRV EQQNTKPNIY DIPKATSSVS
   361  QAGKELEKAK EVSENSAGHN SSWFSRRTTS PSPEPDRLSG SSSDSRASIV SSCSTTSTDD
   421  SSSSSSEESA KELSLDLDVA KETVMALQHK VVSSVAGLML FVSRKWRFRD YLEANIDAIH
   481  RSTDHIEESV REFLDFARGV HGTACNLTDS NLQNRIRDQM QTISNSYRIL LETKESLDNR
   541  NWPLEVLVTD SVQNSPDDLE RFVMVARMLP EDIKRFASIV IANGRLLFKR NCEKEETVQL
   601  TPNAEFKCEK YIQPPQRETE SHQKSTPSTK QREDEHSSEL LKKNRANICG QNPGPLIPQP
   661  SSQQTPERKP RLSEHCRLYF GALFKAISAF HGSLSSSQPA EIITQSKLVI MVGQKLVDTL
   721  CMETQERDVR NEILRGSSHL CSLLKDVALA TKNAVLTYPS PAALGHLQAE AEKLEQHTRQ
   781  FRGTLG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CASS4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.54
Highest tissue expression
11 nTPM

Expression across tissuesHPA

Tissue

  • lung: 11 nTPM
  • appendix: 9.1 nTPM
  • spleen: 5.3 nTPM
  • placenta: 4.2 nTPM
  • lymph node: 3.6 nTPM
  • bone marrow: 2.6 nTPM

Single-cell type

  • neutrophils: 390 nCPM
  • cdc: 92 nCPM
  • t-cells: 81 nCPM
  • innate lymphoid cells: 78 nCPM
  • microglia: 72 nCPM
  • macrophages: 55 nCPM

Immune cell

  • neutrophil: 69 nTPM
  • eosinophil: 23 nTPM
  • myeloid DC: 6.9 nTPM
  • naive CD4 T-cell: 4.2 nTPM
  • classical monocyte: 4 nTPM
  • naive CD8 T-cell: 3.8 nTPM

Brain region

  • medulla oblongata: 5.3 nTPM
  • pons: 4.2 nTPM
  • hypothalamus: 4.1 nTPM
  • thalamus: 4.1 nTPM
  • amygdala: 3.8 nTPM
  • midbrain: 3.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.45
gnomAD pLI
0.37
gnomAD missense Z
1.12
DepMap mean gene effect
-0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CASS4 as an antibody target. Whether an autoantibody or antibody against CASS4 could matter depends on whether native CASS4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CASS4 is annotated at the cell surface, where native CASS4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label CASS4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CASS4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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