Seroatlas · Human Serome Atlas

CARF

Calcium-responsive transcription factor

Also known as: ALS2CR8, CARTF_HUMAN, FLJ21579, NYD-SP24

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N187
Gene: CARF
Ensembl: ENSG00000138380
Chromosome: 2
Canonical length: 725 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli fibrillar center,Vesicles,Cytosol

OverviewNCBI Gene

Enables DNA binding activity and DNA-binding transcription activator activity, RNA polymerase II-specific. Involved in cellular response to calcium ion. Predicted to be located in granular component. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

725 residues, UniProt reviewed canonical sequence.

>Q8N187|CARF
     1  MEQSNDSLRV NHNDGEESKT SAQVFEHLIC MDSRDSSFGQ NDSPTVLPIT TREANNSLIS
    61  QNIPGPLTQT QTLSAEQFHL VDQNGQAIQY ELQSLGESNA QMMIVASPTE NGQVLRVIPP
   121  TQTGMAQVII PQGQLVDVNS PRDVPEEKPS NRNLPTVRVD TLADNTSNYI LHPQTSFPLP
   181  KKSVTGMLEE PLLGPLQPLS SNTPIWACRL RSCEKIGDSY RGYCVSETEL ESVLTFHKQQ
   241  TQSVWGTRQS PSPAKPATRL MWKSQYVPYD GIPFVNAGSR AVVMECQYGP RRKGFQLKKV
   301  SEQESRSCQL YKATCPARIY IKKVQKFPEY RVPTDPKIDK KIIRMEQEKA FNMLKKNLVD
   361  AGGVLRWYVQ LPTQQAHQYH ELETPCLTLS PSPFPVSSLE EEETAVRDEN CALPSRLHPQ
   421  VAHKIQELVS QGIEQVYAVR KQLRKFVERE LFKPDEVPER HNLSFFPTVN DIKNHIHEVQ
   481  KSLRNGDTVY NSEIIPATLQ WTTDSGNILK ETMTVTFAEG NSPGESITTK VETNQTRGSL
   541  SPEPTHLLSS LSSFQPKIFT QLQGLQLQPR YTSPDESPAV VSVNNQPSSS PSGLLDTIGS
   601  AVMNNNSLLL GQSHSLQRDT CLTQNNSTAS TMGNLPEPDQ NLVAMDELVE VGDVEDTGNL
   661  EGTVHRILLG DVQTIPIQII DNHSALIEEN PESTISVSQV KQEPKEPALS MEAKKTVDYK
   721  KLSAT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CARF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 20 nTPM

Expression across tissuesHPA

Tissue

testis: 20 nTPM
retina: 15 nTPM
thyroid gland: 9.9 nTPM
epididymis: 9.4 nTPM
endometrium: 8.8 nTPM
thymus: 8.8 nTPM

Single-cell type

myonuclei: 110 nCPM
adrenal medulla cells: 106 nCPM
oligodendrocytes: 81 nCPM
adipocytes: 73 nCPM
ependymal cells: 71 nCPM
cardiomyocytes: 70 nCPM

Immune cell

NK-cell: 8.9 nTPM
T-reg: 4.7 nTPM
naive CD4 T-cell: 3.1 nTPM
MAIT T-cell: 2.5 nTPM
naive B-cell: 2.4 nTPM
gdT-cell: 2.2 nTPM

Brain region

white matter: 39 nTPM
basal ganglia: 32 nTPM
medulla oblongata: 29 nTPM
pons: 28 nTPM
cerebellum: 28 nTPM
midbrain: 27 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.7
gnomAD pLI: 0
gnomAD missense Z: 1.25
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Calcium-responsive transcription factor
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CARF as an antibody target. Whether an autoantibody or antibody against CARF could matter depends on whether native CARF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CARF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CARF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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