Seroatlas · Human Serome Atlas

CAPZA3

F-actin-capping protein subunit alpha-3

Also known as: CAPPA3, CAZA3_HUMAN, Gsg3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96KX2
Gene: CAPZA3
Ensembl: ENSG00000177938
Chromosome: 12
Canonical length: 299 aa
Protein class: Predicted intracellular proteins
Subcellular location: Calyx

OverviewNCBI Gene

This gene encodes an actin capping protein, one of the F-actin capping protein alpha subunit family. The encoded protein is predominantly localized to the neck region of ejaculated sperm, other immunohistochemical signals were found in the tail and postacrosomal regions. The encoded protein may also form heterodimers of alpha and beta subunits. This protein may be important in determining sperm architecture and male fertility. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

299 residues, UniProt reviewed canonical sequence.

>Q96KX2|CAPZA3
     1  MTLSVLSRKD KERVIRRLLL QAPPGEFVNA FDDLCLLIRD EKLMHHQGEC AGHQHCQKYS
    61  VPLCIDGNPV LLSHHNVMGD YRFFDHQSKL SFKYDLLQNQ LKDIQSHGII QNEAEYLRVV
   121  LLCALKLYVN DHYPKGNCNM LRKTVKSKEY LIACIEDHNY ETGECWNGLW KSKWIFQVNP
   181  FLTQVTGRIF VQAHFFRCVN LHIEISKDLK ESLEIVNQAQ LALSFARLVE EQENKFQAAV
   241  LEELQELSNE ALRKILRRDL PVTRTLIDWH RILSDLNLVM YPKLGYVIYS RSVLCNWII

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CAPZA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.33
Highest tissue expression: 176 nTPM

Expression across tissuesHPA

Tissue

testis: 176 nTPM
skin: 2 nTPM
liver: 1.2 nTPM
stomach: 1.2 nTPM
breast: 0.8 nTPM
kidney: 0.8 nTPM

Single-cell type

late spermatids: 10,439 nCPM
early spermatids: 2,841 nCPM
late primary spermatocytes: 622 nCPM
sertoli cells: 13 nCPM
leydig cells: 6.9 nCPM
peritubular myoid cells: 5.3 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

hypothalamus: 0.5 nTPM
thalamus: 0.2 nTPM
medulla oblongata: 0.1 nTPM
midbrain: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.19
gnomAD pLI: 0
gnomAD missense Z: -0.26
DepMap mean gene effect: 0.14
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

actin filament binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CAPZA3 as an antibody target. Whether an autoantibody or antibody against CAPZA3 could matter depends on whether native CAPZA3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CAPZA3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CAPZA3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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