CAPN9

Protein identityUniProt · HPA

UniProt accession

O14815

Gene

CAPN9

Ensembl

ENSG00000135773

Chromosome

1

Canonical length

690 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Golgi apparatus,Vesicles

OverviewNCBI Gene

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

690 residues, UniProt reviewed canonical sequence.

>O14815|CAPN9
     1  MPYLYRAPGP QAHPVPKDAR ITHSSGQSFE QMRQECLQRG TLFEDADFPA SNSSLFYSER
    61  PQIPFVWKRP GEIVKNPEFI LGGATRTDIC QGELGDCWLL AAIASLTLNQ KALARVIPQD
   121  QSFGPGYAGI FHFQFWQHSE WLDVVIDDRL PTFRDRLVFL HSADHNEFWS ALLEKAYAKL
   181  NGSYEALKGG SAIEAMEDFT GGVAETFQTK EAPENFYEIL EKALKRGSLL GCFIDTRSAA
   241  ESEARTPFGL IKGHAYSVTG IDQVSFRGQR IELIRIRNPW GQVEWNGSWS DSSPEWRSVG
   301  PAEQKRLCHT ALDDGEFWMA FKDFKAHFDK VEICNLTPDA LEEDAIHKWE VTVHQGSWVR
   361  GSTAGGCRNF LDTFWTNPQI KLSLTEKDEG QEECSFLVAL MQKDRRKLKR FGANVLTIGY
   421  AIYECPDKDE HLNKDFFRYH ASRARSKTFI NLREVSDRFK LPPGEYILIP STFEPHQEAD
   481  FCLRIFSEKK AITRDMDGNV DIDLPEPPKP TPPDQETEEE QRFRALFEQV AGEDMEVTAE
   541  ELEYVLNAVL QKKKDIKFKK LSLISCKNII SLMDTSGNGK LEFDEFKVFW DKLKQWINLF
   601  LRFDADKSGT MSTYELRTAL KAAGFQLSSH LLQLIVLRYA DEELQLDFDD FLNCLVRLEN
   661  ASRVFQALST KNKEFIHLNI NEFIHLTMNI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CAPN9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

57 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.14

gnomAD pLI

0

gnomAD missense Z

-0.1

DepMap mean gene effect

-0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• digestion
• proteolysis

Molecular functions

• calcium ion binding
• calcium-dependent cysteine-type endopeptidase activity

Cellular components

• cytoplasm

Protein domainsUniProt · Pfam · InterPro

• Cysteine peptidase, cysteine active site
• Peptidase C2, calpain, catalytic domain
• EF-hand domain
• EF-hand domain pair
• EF-Hand 1, calcium-binding site
• Peptidase C2, calpain, large subunit, domain III
• Peptidase C2, calpain, domain III
• Peptidase C2, calpain family
• Calpain subdomain III
• Calpain large subunit, domain III superfamily
• Papain-like cysteine peptidase superfamily
• Calpain family cysteine protease
• Calpain large subunit, domain III
• EF-hand domain pair

KeywordsUniProt

• Calcium
• Hydrolase
• Metal-binding
• Protease
• Repeat
• Thiol protease