Seroatlas · Human Serome Atlas

CAPN14

Calpain-14

Also known as: CAN14_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A8MX76
Gene: CAPN14
Ensembl: ENSG00000214711
Chromosome: 2
Canonical length: 684 aa
Protein class: Enzymes, Predicted intracellular proteins

OverviewNCBI Gene

Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin-cytoskeletal interactions, and synaptic plasticity (Dear et al., 2000 [PubMed 10964513]). CAPN14 belongs to the calpain large subunit family.[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

684 residues, UniProt reviewed canonical sequence.

>A8MX76|CAPN14
     1  MSLWPPFRCR WKLAPRYSRR ASPQQPQQDF EALLAECLRN GCLFEDTSFP ATLSSIGSGS
    61  LLQKLPPRLQ WKRPPELHSN PQFYFAKAKR LDLCQGIVGD CWFLAALQAL ALHQDILSRV
   121  VPLNQSFTEK YAGIFRFWFW HYGNWVPVVI DDRLPVNEAG QLVFVSSTYK NLFWGALLEK
   181  AYAKLSGSYE DLQSGQVSEA LVDFTGGVTM TINLAEAHGN LWDILIEATY NRTLIGCQTH
   241  SGEKILENGL VEGHAYTLTG IRKVTCKHRP EYLVKLRNPW GKVEWKGDWS DSSSKWELLS
   301  PKEKILLLRK DNDGEFWMTL QDFKTHFVLL VICKLTPGLL SQEAAQKWTY TMREGRWEKR
   361  STAGGQRQLL QDTFWKNPQF LLSVWRPEEG RRSLRPCSVL VSLLQKPRHR CRKRKPLLAI
   421  GFYLYRMNKY HDDQRRLPPE FFQRNTPLSQ PDRFLKEKEV SQELCLEPGT YLIVPCILEA
   481  HQKSEFVLRV FSRKHIFYEI GSNSGVVFSK EIEDQNERQD EFFTKFFEKH PEINAVQLQN
   541  LLNQMTWSSL GSRQPFFSLE ACQGILALLD LNASGTMSIQ EFRDLWKQLK LSQKVFHKQD
   601  RGSGYLNWEQ LHAAMREAGI MLSDDVCQLM LIRYGGPRLQ MDFVSFIHLM LRVENMEDVF
   661  QNLTQDGKGI YLQKPEWMMM ALYS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CAPN14 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 175 nTPM

Expression across tissuesHPA

Tissue

esophagus: 175 nTPM
tonsil: 7.7 nTPM
salivary gland: 2.9 nTPM
vagina: 2.8 nTPM
cervix: 2.6 nTPM
urinary bladder: 0.4 nTPM

Single-cell type

esophageal apical cells: 2,577 nCPM
ocular epithelial cells: 246 nCPM
esophageal suprabasal cells: 236 nCPM
suprabasal keratinocytes: 40 nCPM
urothelial cells: 35 nCPM
esophageal basal cells: 31 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebral cortex: 0.4 nTPM
basal ganglia: 0.3 nTPM
amygdala: 0.2 nTPM
hippocampal formation: 0.2 nTPM
white matter: 0.2 nTPM
cerebellum: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.11
gnomAD pLI: 0
gnomAD missense Z: 1.13
DepMap mean gene effect: 0
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

proteolysis

Molecular functions

Cellular components

cytoplasm

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CAPN14 as an antibody target. Whether an autoantibody or antibody against CAPN14 could matter depends on whether native CAPN14 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CAPN14 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CAPN14 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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