Seroatlas · Human Serome Atlas

C9orf50

Uncharacterized protein C9orf50

Also known as: CI050_HUMAN, FLJ35803

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5SZB4
Gene: C9orf50
Ensembl: ENSG00000179058
Chromosome: 9
Canonical length: 431 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

431 residues, UniProt reviewed canonical sequence.

>Q5SZB4|C9orf50
     1  MFWRRLRPGA QDLAPKGLPG DGDFRRSSDP RLPKLTPPAL RAALGARGSG DWRIPGGGAA
    61  WWPEGDAKPG VGVGRLPPRL PALLTATRRA VRKRGLLRSL LPPPLLSAGA SRESAPRQPG
   121  PGERERPRRR VAREDPDFLG AFLGELLPSR FREFLHQLQE KCAEEPEPLT SPAPQHQRGV
   181  LEHCPGSPRC PNCSFLPDLW GQSSHLQDSL TKISLQQTPI LGPLKGDHSQ FTTVRKANHR
   241  PHGAQVPRLK AALTHNPSGE GSRPCRQRCP FRVRFADETL QDTTLRYWER RRSVQQSVIV
   301  NQKAALPVAS ERVFGSVGKR LESLPKALYP GAKEETLASS SCWDCAGLST QKTQGYLSED
   361  TSMNSSLPFC SWKKAAAQRP RSSLRAFLDP HRNLEQESLL PNRVLQSVLK QGCPKGYHLL
   421  LASATLQPDK R

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against C9orf50 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.7
Highest tissue expression: 7.5 nTPM

Expression across tissuesHPA

Tissue

testis: 7.5 nTPM
amygdala: 1.5 nTPM
hypothalamus: 1.5 nTPM
midbrain: 1.3 nTPM
cerebral cortex: 1.2 nTPM
spinal cord: 1.2 nTPM

Single-cell type

late spermatids: 390 nCPM
early spermatids: 84 nCPM
epididymal efferent duct absorptive cells: 29 nCPM
epididymal efferent duct ciliated cells: 19 nCPM
müller glia: 8.8 nCPM
ependymal cells: 7.4 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

medulla oblongata: 1.5 nTPM
midbrain: 1.1 nTPM
cerebellum: 0.9 nTPM
hypothalamus: 0.9 nTPM
thalamus: 0.9 nTPM
white matter: 0.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.38
gnomAD pLI: 0
gnomAD missense Z: 0.79
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

cytoplasm

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Cytoplasm

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads C9orf50 as an antibody target. Whether an autoantibody or antibody against C9orf50 could matter depends on whether native C9orf50 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

C9orf50 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label C9orf50 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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