BARHL2

Protein identityUniProt · HPA

UniProt accession

Q9NY43

Gene

BARHL2

Ensembl

ENSG00000143032

Chromosome

1

Canonical length

387 aa

Protein class

Predicted intracellular proteins, Transcription factors

OverviewNCBI Gene

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including generation of neurons; positive regulation of macromolecule biosynthetic process; and regulation of axon extension. Predicted to be located in chromatin. Predicted to be active in nucleus. Implicated in oral squamous cell carcinoma. Biomarker of colorectal cancer; oral squamous cell carcinoma; and stomach cancer. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

387 residues, UniProt reviewed canonical sequence.

>Q9NY43|BARHL2
     1  MTMEGASGSS FGIDTILSSA SSGSPGMMNG DFRPLGEART ADFRSQATPS PCSEIDTVGT
    61  APSSPISVTM EPPEPHLVAD ATQHHHHLHH SQQPPPPAAA PTQSLQPLPQ QQQPLPPQQP
   121  PPPPPQQLGS AASAPRTSTS SFLIKDILGD SKPLAACAPY STSVSSPHHT PKQESNAVHE
   181  SFRPKLEQED SKTKLDKRED SQSDIKCHGT KEEGDREITS SRESPPVRAK KPRKARTAFS
   241  DHQLNQLERS FERQKYLSVQ DRMDLAAALN LTDTQVKTWY QNRRTKWKRQ TAVGLELLAE
   301  AGNYSALQRM FPSPYFYHPS LLGSMDSTTA AAAAAAMYSS MYRTPPAPHP QLQRPLVPRV
   361  LIHGLGPGGQ PALNPLSSPI PGTPHPR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BARHL2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.65

Highest tissue expression

42 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.54

gnomAD pLI

0.61

gnomAD missense Z

-0.06

DepMap mean gene effect

-0.17

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• amacrine cell differentiation
• cell fate determination
• neuron migration
• positive regulation of translation
• regulation of axon extension
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II transcription regulatory region sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Homeodomain
• Homedomain-like superfamily
• Homeobox, conserved site
• Homeodomain, metazoa
• Homeodomain
• Mediator complex and homeobox domain-containing protein

KeywordsUniProt

• DNA-binding
• Homeobox
• Nucleus
• Transcription
• Transcription regulation