Seroatlas · Human Serome Atlas

B3GNT5

Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Also known as: B3GN-T5, B3GN5_HUMAN, beta3Gn-T5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9BYG0
Gene: B3GNT5
Ensembl: ENSG00000176597
Chromosome: 3
Canonical length: 378 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoli

OverviewNCBI Gene

This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

378 residues, UniProt reviewed canonical sequence.

>Q9BYG0|B3GNT5
     1  MRMLVSGRRV KKWQLIIQLF ATCFLASLMF FWEPIDNHIV SHMKSYSYRY LINSYDFVND
    61  TLSLKHTSAG PRYQYLINHK EKCQAQDVLL LLFVKTAPEN YDRRSGIRRT WGNENYVRSQ
   121  LNANIKTLFA LGTPNPLEGE ELQRKLAWED QRYNDIIQQD FVDSFYNLTL KLLMQFSWAN
   181  TYCPHAKFLM TADDDIFIHM PNLIEYLQSL EQIGVQDFWI GRVHRGAPPI RDKSSKYYVS
   241  YEMYQWPAYP DYTAGAAYVI SGDVAAKVYE ASQTLNSSLY IDDVFMGLCA NKIGIVPQDH
   301  VFFSGEGKTP YHPCIYEKMM TSHGHLEDLQ DLWKNATDPK VKTISKGFFG QIYCRLMKII
   361  LLCKISYVDT YPCRAAFI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B3GNT5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 72 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 72 nTPM
small intestine: 40 nTPM
duodenum: 36 nTPM
esophagus: 35 nTPM
urinary bladder: 23 nTPM
colon: 21 nTPM

Single-cell type

esophageal apical cells: 652 nCPM
neutrophils: 584 nCPM
ocular epithelial cells: 442 nCPM
urothelial cells: 382 nCPM
esophageal suprabasal cells: 342 nCPM
syncytiotrophoblasts: 321 nCPM

Immune cell

neutrophil: 5.6 nTPM
classical monocyte: 1 nTPM
eosinophil: 1 nTPM
myeloid DC: 0.7 nTPM
total PBMC: 0.3 nTPM
T-reg: 0.2 nTPM

Brain region

medulla oblongata: 20 nTPM
white matter: 19 nTPM
spinal cord: 17 nTPM
hypothalamus: 16 nTPM
pons: 16 nTPM
midbrain: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.49
gnomAD pLI: 0.73
gnomAD missense Z: 1.05
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

glycosyltransferase activity
lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity

Cellular components

Golgi membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads B3GNT5 as an antibody target. Whether an autoantibody or antibody against B3GNT5 could matter depends on whether native B3GNT5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

B3GNT5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label B3GNT5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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