B3GAT1

Protein identityUniProt · HPA

UniProt accession

Q9P2W7

Gene

B3GAT1

Ensembl

ENSG00000109956

Chromosome

11

Canonical length

334 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted secreted proteins

Subcellular location

Vesicles

Secretome location

Secreted in brain

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

334 residues, UniProt reviewed canonical sequence.

>Q9P2W7|B3GAT1
     1  MPKRRDILAI VLIVLPWTLL ITVWHQSTLA PLLAVHKDEG SDPRRETPPG ADPREYCTSD
    61  RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA ELTRMANTLL HVPNLHWLVV
   121  EDAPRRTPLT ARLLRDTGLN YTHLHVETPR NYKLRGDARD PRIPRGTMQR NLALRWLRET
   181  FPRNSSQPGV VYFADDDNTY SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG
   241  WKTVFDPHRP FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK
   301  AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B3GAT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

191 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for B3GAT1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.64

gnomAD pLI

0.14

gnomAD missense Z

2.42

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• carbohydrate metabolic process
• chondroitin sulfate proteoglycan biosynthetic process
• glycosaminoglycan biosynthetic process
• visual learning

Molecular functions

• galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
• metal ion binding

Cellular components

• endoplasmic reticulum membrane
• extracellular region
• Golgi membrane
• membrane

Protein domainsUniProt · Pfam · InterPro

• Glycosyl transferase, family 43
• Nucleotide-diphospho-sugar transferases
• Glycosyltransferase family 43

KeywordsUniProt

• Endoplasmic reticulum
• Glycoprotein
• Golgi apparatus
• Manganese
• Membrane
• Metal-binding
• Phosphoprotein
• Secreted
• Signal-anchor
• Transferase
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of B3GAT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SAR1A