Seroatlas · Human Serome Atlas

B3GALT4

Beta-1,3-galactosyltransferase 4

Also known as: B3GT4_HUMAN, beta3Gal-T4, GalT4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O96024
Gene: B3GALT4
Ensembl: ENSG00000235863
Chromosome: 6
Canonical length: 378 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

378 residues, UniProt reviewed canonical sequence.

>O96024|B3GALT4
     1  MQLRLFRRLL LAALLLVIVW TLFGPSGLGE ELLSLSLASL LPAPASPGPP LALPRLLIPN
    61  QEACSGPGAP PFLLILVCTA PENLNQRNAI RASWGGLREA RGLRVQTLFL LGEPNAQHPV
   121  WGSQGSDLAS ESAAQGDILQ AAFQDSYRNL TLKTLSGLNW AEKHCPMARY VLKTDDDVYV
   181  NVPELVSELV LRGGRWGQWE RSTEPQREAE QEGGQVLHSE EVPLLYLGRV HWRVNPSRTP
   241  GGRHRVSEEQ WPHTWGPFPP YASGTGYVLS ASAVQLILKV ASRAPLLPLE DVFVGVSARR
   301  GGLAPTQCVK LAGATHYPLD RCCYGKFLLT SHRLDPWKMQ EAWKLVGGSD GERTAPFCSW
   361  FQGVLGILRC RAIAWLQS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B3GALT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

testis: 26 nTPM
esophagus: 19 nTPM
skin: 15 nTPM
blood vessel: 13 nTPM
spleen: 12 nTPM
salivary gland: 12 nTPM

Single-cell type

papillary tip epithelial cells: 15 nCPM
podocytes: 14 nCPM
renal collecting duct principal cells: 11 nCPM
microglia: 7.2 nCPM
bergmann glia: 6.5 nCPM
oligodendrocytes: 6.2 nCPM

Immune cell

naive B-cell: 8.9 nTPM
eosinophil: 7.7 nTPM
non-classical monocyte: 5.7 nTPM
NK-cell: 5 nTPM
plasmacytoid DC: 4.7 nTPM
gdT-cell: 4.6 nTPM

Brain region

cerebellum: 8.6 nTPM
medulla oblongata: 8.3 nTPM
white matter: 7.5 nTPM
pons: 7.3 nTPM
cerebral cortex: 6.9 nTPM
thalamus: 6.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.74
gnomAD pLI: 0.03
gnomAD missense Z: 1.45
DepMap mean gene effect: -0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

glycosyltransferase activity
N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity
ganglioside galactosyltransferase activity

Cellular components

Golgi membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads B3GALT4 as an antibody target. Whether an autoantibody or antibody against B3GALT4 could matter depends on whether native B3GALT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

B3GALT4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label B3GALT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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