ATP6V0E1

Protein identityUniProt · HPA

UniProt accession

O15342

Gene

ATP6V0E1

Ensembl

ENSG00000113732

Chromosome

5

Canonical length

81 aa

Protein class

Metabolic proteins, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c"""""""""""""""""""""""""""""""", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

81 residues, UniProt reviewed canonical sequence.

>O15342|ATP6V0E1
     1  MAYHGLTVPL IVMSVFWGFV GFLVPWFIPK GPNRGVIITM LVTCSVCCYL FWLIAILAQL
    61  NPLFGPQLKN ETIWYLKYHW P

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ATP6V0E1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

2

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

598 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.94

gnomAD pLI

0.4

gnomAD missense Z

0.14

DepMap mean gene effect

-0.12

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• proton transmembrane transport
• regulation of macroautophagy
• transmembrane transport
• vacuolar acidification

Molecular functions

• ATPase-coupled ion transmembrane transporter activity
• proton-transporting ATPase activity, rotational mechanism

Cellular components

• endosome membrane
• lysosomal membrane
• phagocytic vesicle membrane
• proton-transporting V-type ATPase, V0 domain

Protein domainsUniProt · Pfam · InterPro

• ATPase, V0 complex, subunit e1/e2
• ATPase, V0 complex, subunit e1/e2, metazoa
• ATP synthase subunit H

KeywordsUniProt

• Glycoprotein
• Hydrogen ion transport
• Ion transport
• Membrane
• Transmembrane
• Transmembrane helix
• Transport