ATP5MF

Protein identityUniProt · HPA

UniProt accession

P56134

Gene

ATP5MF

Ensembl

ENSG00000241468

Chromosome

7

Canonical length

94 aa

Protein class

Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters

Subcellular location

Nuclear membrane,Mitochondria

Quaternary structure

Homooctamer

OverviewNCBI Gene

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene. [provided by RefSeq, Nov 2010]

Canonical amino-acid sequenceUniProt

94 residues, UniProt reviewed canonical sequence.

>P56134|ATP5MF
     1  MASVGECPAP VPVKDKKLLE VKLGELPSWI LMRDFSPSGI FGAFQRGYYR YYNKYINVKK
    61  GSISGITMVL ACYVLFSYSF SYKHLKHERL RKYH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ATP5MF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.53

Highest tissue expression

1,343 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.5

gnomAD pLI

0.02

DepMap mean gene effect

-1.03

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• proton motive force-driven ATP synthesis
• proton motive force-driven mitochondrial ATP synthesis
• proton transmembrane transport

Cellular components

• mitochondrial inner membrane
• mitochondrion
• nuclear membrane
• proton-transporting ATP synthase complex

Protein domainsUniProt · Pfam · InterPro

• Mitochondrial F1-F0 ATP synthase subunit F, predicted
• Mitochondrial F1F0-ATP synthase, subunit f

KeywordsUniProt

• Acetylation
• ATP synthesis
• CF(0)
• Hydrogen ion transport
• Ion transport
• Membrane
• Mitochondrion
• Mitochondrion inner membrane
• Phosphoprotein
• Transmembrane
• Transmembrane helix
• Transport