Seroatlas · Human Serome Atlas

ART4

Ecto-ADP-ribosyltransferase 4

Also known as: ARTC4, CD297, DO, DOK1, NAR4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q93070
Gene: ART4
Ensembl: ENSG00000111339
Chromosome: 12
Canonical length: 314 aa
Protein class: Blood group antigen proteins, CD markers, Enzymes, Metabolic proteins, Predicted membrane proteins, Predicted secreted proteins
Subcellular location: Rods & Rings
Secretome location: Secreted to blood

OverviewNCBI Gene

This gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

314 residues, UniProt reviewed canonical sequence.

>Q93070|ART4
     1  MGPLINRCKK ILLPTTVPPA TMRIWLLGGL LPFLLLLSGL QRPTEGSEVA IKIDFDFAPG
    61  SFDDQYQGCS KQVMEKLTQG DYFTKDIEAQ KNYFRMWQKA HLAWLNQGKV LPQNMTTTHA
   121  VAILFYTLNS NVHSDFTRAM ASVARTPQQY ERSFHFKYLH YYLTSAIQLL RKDSIMENGT
   181  LCYEVHYRTK DVHFNAYTGA TIRFGQFLST SLLKEEAQEF GNQTLFTIFT CLGAPVQYFS
   241  LKKEVLIPPY ELFKVINMSY HPRGDWLQLR STGNLSTYNC QLLKASSKKC IPDPIAIASL
   301  SFLTSVIIFS KSRV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ART4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 50 nTPM

Expression across tissuesHPA

Tissue

liver: 50 nTPM
spleen: 18 nTPM
blood vessel: 9.2 nTPM
heart muscle: 8.7 nTPM
gallbladder: 8.4 nTPM
lymph node: 8 nTPM

Single-cell type

lymphatic endothelial cells: 108 nCPM
hepatocytes: 86 nCPM
hepatic stellate cells: 78 nCPM
erythrocyte progenitors: 41 nCPM
rod photoreceptor cells: 29 nCPM
cardiomyocytes: 27 nCPM

Immune cell

basophil: 0.1 nTPM
naive CD8 T-cell: 0.1 nTPM
neutrophil: 0.1 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM

Brain region

hypothalamus: 3.4 nTPM
medulla oblongata: 2.3 nTPM
white matter: 2.3 nTPM
basal ganglia: 2.1 nTPM
pons: 2.1 nTPM
choroid plexus: 2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.07
gnomAD pLI: 0
gnomAD missense Z: -0.14
DepMap mean gene effect: 0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

arginine metabolic process

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ART4 as an antibody target. Whether an autoantibody or antibody against ART4 could matter depends on whether native ART4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ART4 is annotated at the cell surface, where native ART4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ART4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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