Seroatlas · Human Serome Atlas

ARHGAP8

Rho GTPase-activating protein 8

Also known as: BPGAP1, FLJ20185, RHG08_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P85298
Gene: ARHGAP8
Ensembl: ENSG00000241484
Chromosome: 22
Canonical length: 464 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active (GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomain protein that functions to promote Erk activation and cell motility. Alternative splicing results in multiple transcript variants. Read-through transcripts from the upstream proline rich 5, renal (PRR5) gene into this gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]

Canonical amino-acid sequenceUniProt

464 residues, UniProt reviewed canonical sequence.

>P85298|ARHGAP8
     1  MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD HQRLLEYLKY
    61  TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK DGDLTMWPRL VSNSKLKRSS
   121  HLSLPKYWDY RYKKNLKALY VVHPTSFIKV LWNILKPLIS HKFGKKVIYF NYLSELHEHL
   181  KYDQLVIPPE VLRYDEKLQS LHEGRTPPPT KTPPPRPPLP TQQFGVSLQY LKDKNQGELI
   241  PPVLRFTVTY LREKGLRTEG LFRRSASVQT VREIQRLYNQ GKPVNFDDYG DIHIPAVILK
   301  TFLRELPQPL LTFQAYEQIL GITCVESSLR VTGCRQILRS LPEHNYVVLR YLMGFLHAVS
   361  RESIFNKMNS SNLACVFGLN LIWPSQGVSS LSALVPLNMF TELLIEYYEK IFSTPEAPGE
   421  HGLAPWEQGS RAAPLQEAVP RTQATGLTKP TLPPSPLMAA RRRL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

kidney: 23 nTPM
salivary gland: 22 nTPM
thyroid gland: 15 nTPM
prostate: 11 nTPM
epididymis: 11 nTPM
colon: 8.9 nTPM

Single-cell type

choroid plexus epithelial cells: 64 nCPM
oocytes: 41 nCPM
paneth cells: 16 nCPM
enteric stem cells: 13 nCPM
bergmann glia: 13 nCPM
enteric transient amplifying cells: 12 nCPM

Immune cell

T-reg: 0.3 nTPM
plasmacytoid DC: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM

Brain region

choroid plexus: 12 nTPM
cerebellum: 4.6 nTPM
cerebral cortex: 4.1 nTPM
thalamus: 3.7 nTPM
basal ganglia: 3.4 nTPM
pons: 3.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.75
gnomAD pLI: 0
gnomAD missense Z: -4.19
DepMap mean gene effect: 0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

GTPase activator activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

GTPase activation

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGAP8 as an antibody target. Whether an autoantibody or antibody against ARHGAP8 could matter depends on whether native ARHGAP8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGAP8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGAP8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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