Seroatlas · Human Serome Atlas

ARHGAP28

Rho GTPase-activating protein 28

Also known as: FLJ10312, KIAA1314, RHG28_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9P2N2
Gene
ARHGAP28
Ensembl
ENSG00000088756
Chromosome
18
Canonical length
729 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cell Junctions

OverviewNCBI Gene

Predicted to enable GTPase activator activity. Predicted to be involved in negative regulation of stress fiber assembly; regulation of actin filament polymerization; and regulation of small GTPase mediated signal transduction. Located in cell junction and nucleoplasm. Implicated in allergic disease. Biomarker of meningioma. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

729 residues, UniProt reviewed canonical sequence.

>Q9P2N2|ARHGAP28
     1  MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI NRMLSNESLH
    61  PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP PAEVTPVDEG ELEAEWLQDV
   121  GLSTLISGDE EEDGKALLST LTRTQAAAVQ KRYHTYTQTM RKKDKQSIRD VRDIFGVSES
   181  PPRDTCGNHT NQLDGTKEER ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD
   241  SVAILETIPV LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL
   301  KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE LTAFFDAFGI
   361  QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL VLQKFFEKVE ESGLESEGIF
   421  RLSGCTAKVK QYREELDAKF NADKFKWDKM CHREAAVMLK AFFRELPTSL FPVEYIPAFI
   481  SLMERGPHVK VQFQALHLMV MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA
   541  PNLFFSRSKH SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ
   601  LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL SKVSMAIQLN
   661  NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE HCLDPDAYIL DVYRINPQAE
   721  WVIKPQQSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
72 nTPM

Expression across tissuesHPA

Tissue

  • testis: 72 nTPM
  • placenta: 24 nTPM
  • spleen: 12 nTPM
  • epididymis: 8.2 nTPM
  • cervix: 8.1 nTPM
  • kidney: 7.9 nTPM

Single-cell type

  • podocytes: 1,006 nCPM
  • late spermatids: 704 nCPM
  • early spermatids: 623 nCPM
  • late primary spermatocytes: 255 nCPM
  • mesothelial cells: 234 nCPM
  • prostatic glandular cells: 139 nCPM

Immune cell

  • neutrophil: 2.9 nTPM
  • basophil: 0.6 nTPM
  • eosinophil: 0.3 nTPM
  • classical monocyte: 0.2 nTPM
  • naive B-cell: 0.2 nTPM
  • plasmacytoid DC: 0.2 nTPM

Brain region

  • cerebellum: 11 nTPM
  • hypothalamus: 11 nTPM
  • cerebral cortex: 11 nTPM
  • basal ganglia: 10 nTPM
  • hippocampal formation: 10 nTPM
  • amygdala: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.86
gnomAD pLI
0
gnomAD missense Z
0.78
DepMap mean gene effect
0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGAP28 as an antibody target. Whether an autoantibody or antibody against ARHGAP28 could matter depends on whether native ARHGAP28 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGAP28 is annotated at the cell surface, where native ARHGAP28 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ARHGAP28 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARHGAP28. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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