Seroatlas · Human Serome Atlas

ARHGAP25

Rho GTPase-activating protein 25

Also known as: KIAA0053, RHG25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P42331
Gene
ARHGAP25
Ensembl
ENSG00000163219
Chromosome
2
Canonical length
645 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles

OverviewNCBI Gene

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

645 residues, UniProt reviewed canonical sequence.

>P42331|ARHGAP25
     1  MSLKLPRNWD FNLKVEAAKI ARSRSVMTGE QMAAFHPSST PNPLERPIKM GWLKKQRSIV
    61  KNWQQRYFVL RAQQLYYYKD EEDTKPQGCM YLPGCTIKEI ATNPEEAGKF VFEIIPASWD
   121  QNRMGQDSYV LMASSQAEME EWVKFLRRVA GTPCGVFGQR LDETVAYEQK FGPHLVPILV
   181  EKCAEFILEH GRNEEGIFRL PGQDNLVKQL RDAFDAGERP SFDRDTDVHT VASLLKLYLR
   241  DLPEPVVPWS QYEGFLLCGQ LTNADEAKAQ QELMKQLSIL PRDNYSLLSY ICRFLHEIQL
   301  NCAVNKMSVD NLATVIGVNL IRSKVEDPAV IMRGTPQIQR VMTMMIRDHE VLFPKSKDIP
   361  LSPPAQKNDP KKAPVARSSV GWDATEDLRI SRTDSFSSMT SDSDTTSPTG QQPSDAFPED
   421  SSKVPREKPG DWKMQSRKRT QTLPNRKCFL TSAFQGANSS KMEIFKNEFW SPSSEAKAGE
   481  GHRRTMSQDL RQLSDSQRTS TYDNVPSLPG SPGEEASALS SQACDSKGDT LASPNSETGP
   541  GKKNSGEEEI DSLQRMVQEL RKEIETQKQM YEEQIKNLEK ENYDVWAKVV RLNEELEKEK
   601  KKSAALEISL RNMERSREDV EKRNKALEEE VKEFVKSMKE PKTEA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.48
Highest tissue expression
71 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 71 nTPM
  • lymph node: 67 nTPM
  • tonsil: 63 nTPM
  • appendix: 37 nTPM
  • bone marrow: 35 nTPM
  • thymus: 26 nTPM

Single-cell type

  • neutrophils: 508 nCPM
  • microglia: 414 nCPM
  • thymocytes: 303 nCPM
  • b-cells: 277 nCPM
  • mast cells: 211 nCPM
  • neutrophil progenitors: 202 nCPM

Immune cell

  • neutrophil: 660 nTPM
  • eosinophil: 530 nTPM
  • basophil: 236 nTPM
  • gdT-cell: 172 nTPM
  • NK-cell: 171 nTPM
  • memory B-cell: 157 nTPM

Brain region

  • white matter: 11 nTPM
  • medulla oblongata: 8.7 nTPM
  • spinal cord: 7.8 nTPM
  • thalamus: 7.3 nTPM
  • pons: 6 nTPM
  • midbrain: 5.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.34
gnomAD pLI
0.95
gnomAD missense Z
0.62
DepMap mean gene effect
0.08
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGAP25 as an antibody target. Whether an autoantibody or antibody against ARHGAP25 could matter depends on whether native ARHGAP25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGAP25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGAP25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARHGAP25. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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