Seroatlas · Human Serome Atlas

APEH

Acylamino-acid-releasing enzyme

Also known as: ACPH_HUMAN, D3F15S2, D3S48E, DNF15S2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P13798
Gene
APEH
Ensembl
ENSG00000164062
Chromosome
3
Canonical length
732 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Cytosol
Quaternary structure
Homotetramer

OverviewNCBI Gene

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

732 residues, UniProt reviewed canonical sequence.

>P13798|APEH
     1  MERQVLLSEP EEAAALYRGL SRQPALSAAC LGPEVTTQYG GQYRTVHTEW TQRDLERMEN
    61  IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG TMKAVLRKAG GTGPGEEKQF
   121  LEVWEKNRKL KSFNLSALEK HGPVYEDDCF GCLSWSHSET HLLYVAEKKR PKAESFFQTK
   181  ALDVSASDDE IARLKKPDQA IKGDQFVFYE DWGENMVSKS IPVLCVLDVE SGNISVLEGV
   241  PENVSPGQAF WAPGDAGVVF VGWWHEPFRL GIRFCTNRRS ALYYVDLIGG KCELLSDDSL
   301  AVSSPRLSPD QCRIVYLQYP SLIPHHQCSQ LCLYDWYTKV TSVVVDVVPR QLGENFSGIY
   361  CSLLPLGCWS ADSQRVVFDS AQRSRQDLFA VDTQVGTVTS LTAGGSGGSW KLLTIDQDLM
   421  VAQFSTPSLP PTLKVGFLPS AGKEQSVLWV SLEEAEPIPD IHWGIRVLQP PPEQENVQYA
   481  GLDFEAILLQ PGSPPDKTQV PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS
   541  TGFGQDSILS LPGNVGHQDV KDVQFAVEQV LQEEHFDASH VALMGGSHGG FISCHLIGQY
   601  PETYRACVAR NPVINIASML GSTDIPDWCV VEAGFPFSSD CLPDLSVWAE MLDKSPIRYI
   661  PQVKTPLLLM LGQEDRRVPF KQGMEYYRAL KTRNVPVRLL LYPKSTHALS EVEVESDSFM
   721  NAVLWLRTHL GS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against APEH can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.23
Highest tissue expression
97 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 97 nTPM
  • tongue: 97 nTPM
  • kidney: 61 nTPM
  • liver: 59 nTPM
  • parathyroid gland: 59 nTPM
  • rectum: 49 nTPM

Single-cell type

  • cytotrophoblasts: 100 nCPM
  • migrating cytotrophoblasts: 85 nCPM
  • colonocytes: 80 nCPM
  • hepatocytes: 80 nCPM
  • enterocytes: 76 nCPM
  • esophageal suprabasal cells: 76 nCPM

Immune cell

  • basophil: 58 nTPM
  • eosinophil: 53 nTPM
  • total PBMC: 52 nTPM
  • T-reg: 51 nTPM
  • non-classical monocyte: 48 nTPM
  • intermediate monocyte: 48 nTPM

Brain region

  • choroid plexus: 55 nTPM
  • pons: 48 nTPM
  • midbrain: 42 nTPM
  • hypothalamus: 38 nTPM
  • thalamus: 38 nTPM
  • medulla oblongata: 37 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.83
gnomAD pLI
0
gnomAD missense Z
1.21
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads APEH as an antibody target. Whether an autoantibody or antibody against APEH could matter depends on whether native APEH is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

APEH is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label APEH as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/APEH. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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