ANXA5

Protein identityUniProt · HPA

UniProt accession

P08758

Gene

ANXA5

Ensembl

ENSG00000164111

Chromosome

4

Canonical length

320 aa

Protein class

Disease related genes, FDA approved drug targets, Plasma proteins, Predicted intracellular proteins, Transporters

Subcellular location

Nuclear membrane

OverviewNCBI Gene

The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications. [provided by RefSeq, Dec 2019]

Canonical amino-acid sequenceUniProt

320 residues, UniProt reviewed canonical sequence.

>P08758|ANXA5
     1  MAQVLRGTVT DFPGFDERAD AETLRKAMKG LGTDEESILT LLTSRSNAQR QEISAAFKTL
    61  FGRDLLDDLK SELTGKFEKL IVALMKPSRL YDAYELKHAL KGAGTNEKVL TEIIASRTPE
   121  ELRAIKQVYE EEYGSSLEDD VVGDTSGYYQ RMLVVLLQAN RDPDAGIDEA QVEQDAQALF
   181  QAGELKWGTD EEKFITIFGT RSVSHLRKVF DKYMTISGFQ IEETIDRETS GNLEQLLLAV
   241  VKSIRSIPAY LAETLYYAMK GAGTDDHTLI RVMVSRSEID LFNIRKEFRK NFATSLYSMI
   301  KGDTSGDYKK ALLLLCGEDD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANXA5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

460 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ANXA5.

Disease | AllUniProt

Conditions ANXA5 is implicated in, by any mechanism.

• Pregnancy loss, recurrent, 3 (RPRGL3) MIM:614391

Disease | AutoantibodyPubMed

Conditions in which antibodies against ANXA5 are reported. Each links to that disease's full target list.

• Antiphospholipid Syndrome 39
• Abortion, Habitual 15
• Thrombosis 14
• Lupus Erythematosus, Systemic 13
• Abortion, Spontaneous 11
• Venous Thrombosis 5
• Stroke 4
• COVID-19 3
• Myocardial Infarction 3
• Pre-Eclampsia 3

Showing 10 of 12 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for ANXA5 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.45

gnomAD pLI

0

gnomAD missense Z

0.55

DepMap mean gene effect

0.08

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• blood coagulation
• negative regulation of apoptotic process
• negative regulation of coagulation
• signal transduction

Molecular functions

• calcium ion binding
• calcium-dependent phospholipid binding
• phosphatidylserine binding
• phospholipase inhibitor activity
• phospholipid binding

Cellular components

• cytoplasm
• cytosol
• endothelial microparticle
• external side of plasma membrane
• extracellular exosome
• extracellular matrix
• extracellular region
• focal adhesion
• membrane
• sarcolemma
• vesicle membrane

Protein domainsUniProt · Pfam · InterPro

• Annexin
• Annexin repeat, conserved site
• Annexin repeat
• Annexin superfamily
• Annexin
• Annexin A5

KeywordsUniProt

• Acetylation
• Annexin
• Blood coagulation
• Calcium
• Calcium/phospholipid-binding
• Hemostasis
• Isopeptide bond
• Phosphoprotein
• Repeat
• S-nitrosylation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of ANXA5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• EIF5B