Seroatlas · Human Serome Atlas

ANKZF1

tRNA endonuclease ANKZF1

Also known as: ANKZ1_HUMAN, FLJ10415, Vms1, ZNF744

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H8Y5
Gene: ANKZF1
Ensembl: ENSG00000163516
Chromosome: 2
Canonical length: 726 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies,Cytosol

OverviewNCBI Gene

Enables RNA endonuclease activity and catalytic activity, acting on a tRNA. Involved in cellular response to hydrogen peroxide; protein quality control for misfolded or incompletely synthesized proteins; and rescue of stalled ribosome. Located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

726 residues, UniProt reviewed canonical sequence.

>Q9H8Y5|ANKZF1
     1  MSPAPDAAPA PASISLFDLS ADAPVFQGLS LVSHAPGEAL ARAPRTSCSG SGERESPERK
    61  LLQGPMDISE KLFCSTCDQT FQNHQEQREH YKLDWHRFNL KQRLKDKPLL SALDFEKQSS
   121  TGDLSSISGS EDSDSASEED LQTLDRERAT FEKLSRPPGF YPHRVLFQNA QGQFLYAYRC
   181  VLGPHQDPPE EAELLLQNLQ SRGPRDCVVL MAAAGHFAGA IFQGREVVTH KTFHRYTVRA
   241  KRGTAQGLRD ARGGPSHSAG ANLRRYNEAT LYKDVRDLLA GPSWAKALEE AGTILLRAPR
   301  SGRSLFFGGK GAPLQRGDPR LWDIPLATRR PTFQELQRVL HKLTTLHVYE EDPREAVRLH
   361  SPQTHWKTVR EERKKPTEEE IRKICRDEKE ALGQNEESPK QGSGSEGEDG FQVELELVEL
   421  TVGTLDLCES EVLPKRRRRK RNKKEKSRDQ EAGAHRTLLQ QTQEEEPSTQ SSQAVAAPLG
   481  PLLDEAKAPG QPELWNALLA ACRAGDVGVL KLQLAPSPAD PRVLSLLSAP LGSGGFTLLH
   541  AAAAAGRGSV VRLLLEAGAD PTVQDSRARP PYTVAADKST RNEFRRFMEK NPDAYDYNKA
   601  QVPGPLTPEM EARQATRKRE QKAARRQREE QQQRQQEQEE REREEQRRFA ALSDREKRAL
   661  AAERRLAAQL GAPTSPIPDS AIVNTRRCWS CGASLQGLTP FHYLDFSFCS TRCLQDHRRQ
   721  AGRPSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKZF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 24 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 24 nTPM
choroid plexus: 18 nTPM
spleen: 18 nTPM
skin: 17 nTPM
skeletal muscle: 16 nTPM
ovary: 16 nTPM

Single-cell type

esophageal apical cells: 63 nCPM
tuft cells: 58 nCPM
respiratory ciliated cells: 56 nCPM
breast lactating cells: 39 nCPM
extravillous trophoblasts: 38 nCPM
fallopian tube ciliated cells: 38 nCPM

Immune cell

NK-cell: 25 nTPM
naive CD4 T-cell: 19 nTPM
naive CD8 T-cell: 18 nTPM
MAIT T-cell: 17 nTPM
T-reg: 17 nTPM
memory CD4 T-cell: 16 nTPM

Brain region

white matter: 14 nTPM
medulla oblongata: 14 nTPM
cerebellum: 13 nTPM
choroid plexus: 13 nTPM
cerebral cortex: 12 nTPM
pons: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.24
gnomAD pLI: 0
gnomAD missense Z: -0.24
DepMap mean gene effect: -0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Ankyrin repeat
Zinc finger C2H2-type
Ankyrin repeat-containing domain superfamily
Ankyrin repeat
VLRF1/Vms1
Vms1-associating treble clef domain
tRNA endonuclease ANKZF1/VMS1
Vms1-associating treble clef domain
Bacteroidetes VLRF1 release factor

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANKZF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKZF1 as an antibody target. Whether an autoantibody or antibody against ANKZF1 could matter depends on whether native ANKZF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKZF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKZF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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