Seroatlas · Human Serome Atlas

ANKRD35

Ankyrin repeat domain-containing protein 35

Also known as: ANR35_HUMAN, FLJ25124

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N283
Gene: ANKRD35
Ensembl: ENSG00000198483
Chromosome: 1
Canonical length: 1001 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles,Plasma membrane,Primary cilium,Primary cilium transition zone,Basal body

OverviewNCBI Gene

Predicted to enable actin binding activity. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1001 residues, UniProt reviewed canonical sequence.

>Q8N283|ANKRD35
     1  MKRIFSCSST QVAVERWNRH DQKLLEAVHR GDVGRVAALA SRKSARPTKL DSNGQSPFHL
    61  AASKGLTECL TILLANGADI NSKNEDGSTA LHLATISCQP QCVKVLLQHG ANEDAVDAEN
   121  RSPLHWAASS GCASSVLLLC DHEAFLDVLD NDGRTPLMIA SLGGHAAICS QLLQRGARVN
   181  VTDKNDKSAL ILACEKGSAE VAELLLSHGA DAGAVDSTGH DALHYALHTQ DKALWRHLQQ
   241  ALSRRRRGGQ RLVQHPDLAS QASPSEPQAG SPPKSSWRAE PEEEQEEKED EDPCSEEWRW
   301  KYEEERRKVV RLEQELVQKT EECKTQAAAY LDLENQIREQ AQELGVLLSW EPRASGKQGS
   361  SLRPGGDGME QGCPKDLLAE STQELKKQQQ AAATVNPVLA PKKAEDSAPG KIQYEVHGRS
   421  QPEEQGPPQS PASETIRKAT GQQLTTNGAQ TFGPDHADQL PAGQKESSQV LGVEPGGTVA
   481  EPVGPAAMNQ LLLQLREELA AVWREKDAAR GALSRPVMEG ALGTPRAEAA AAAWEKMEAR
   541  LERVLARLEW AKAGLQVKPE VPSQESREGA LKAAPGSIKQ DEEKEKRVPG AQGEPLGALG
   601  GEKALGGLAK GQLEKEMSVL RLSNSNLLEE LGELGRERQR LQRELQSLSQ RLQREFVPKP
   661  QAQVQLQQLR QSVGLLTNEL AMEKEATEKL RKLLASQSSG LRGLWDCLPA DLVGERSAQS
   721  KAAESLEELR ACISTLVDRH REAQQVLARL QEENQQLRGS LSPCREPGTS LKAPASPQVA
   781  ALEQDLGKLE EELRAVQATM SGKSQEIGKL KQLLYQATEE VAELRAREAA SLRQHEKTRG
   841  SLVAQAQAWG QELKALLEKY NTACREVGRL REAVAEERRR SGDLAAQAAE QERQASEMRG
   901  RSEQFEKTAE LLKEKMEHLI GACRDKEAKI KELLKKLEQL SEEVLAIRGE NARLALQLQD
   961  SQKNHEEIIS TYRNHLLNAA RGYMEHEVYN ILLQILSMEE E

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKRD35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 58 nTPM

Expression across tissuesHPA

Tissue

skin: 58 nTPM
esophagus: 24 nTPM
vagina: 20 nTPM
cervix: 19 nTPM
blood vessel: 11 nTPM
salivary gland: 11 nTPM

Single-cell type

esophageal apical cells: 113 nCPM
endometrial ciliated cells: 92 nCPM
esophageal suprabasal cells: 69 nCPM
suprabasal keratinocytes: 45 nCPM
peritubular myoid cells: 41 nCPM
respiratory secretory cells: 41 nCPM

Immune cell

classical monocyte: 2.1 nTPM
memory CD8 T-cell: 1.1 nTPM
intermediate monocyte: 0.8 nTPM
gdT-cell: 0.7 nTPM
naive CD8 T-cell: 0.7 nTPM
NK-cell: 0.7 nTPM

Brain region

midbrain: 9.5 nTPM
thalamus: 8.5 nTPM
medulla oblongata: 7.7 nTPM
hypothalamus: 7.6 nTPM
pons: 7.4 nTPM
spinal cord: 7.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.08
gnomAD pLI: 0
gnomAD missense Z: 0.32
DepMap mean gene effect: -0.26
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

actin binding

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKRD35 as an antibody target. Whether an autoantibody or antibody against ANKRD35 could matter depends on whether native ANKRD35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKRD35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKRD35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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