Seroatlas · Human Serome Atlas

ANKFN1

Ankyrin repeat and fibronectin type-III domain-containing protein 1

Also known as: ANKF1_HUMAN, FLJ38335

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N957
Gene: ANKFN1
Ensembl: ENSG00000153930
Chromosome: 17
Canonical length: 1146 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Plasma membrane

OverviewNCBI Gene

Predicted to be involved in establishment of mitotic spindle orientation and regulation of establishment of bipolar cell polarity. Predicted to act upstream of or within behavioral fear response; equilibrioception; and locomotor rhythm. Predicted to be active in spindle. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1146 residues, UniProt reviewed canonical sequence.

>Q8N957|ANKFN1
     1  MNEKRLLFKD RHFTCSKIIG RRFACFAQRL SHRRKQSQCD LLNESTGQLP TTCSSAASNS
    61  INWNCRVKMT QQMQNLHLCQ SKKHSAPSSP NAAKRLYRNL SEKLKGSHSS FDEAYFRTRT
   121  DRLSLRKTSV NFQGNEAMFE AVEQQDMDAV QILLYQYTPE ELDLNTPNSE GLTPLDIAIM
   181  TNNVPIARIL LRTGARESPH FVSLESRAMH LNTLVQEAQE RVSELSAQVE NEGFTLDNTE
   241  KEKQLKAWEW RYRLYRRMKT GFEHARAPEM PTNVCLMVTS STSLTVSFQE PLSVNAAVVT
   301  RYKVEWSMSE DFSPLAGEII MDNLQTLRCT ITGLTMGQQY FVQVSAYNMK GWGPAQTTTP
   361  ACASPSNWKD YDDREPRHKG QSEVLEGLLQ QVRALHQHYS CRESTKLQTT GRKQSVSRSL
   421  KHLFHSSNKF VKTLKRGLYI AVIFYYKDNI LVTNEDQVPI VEIDDSHTSS ITQDFLWFTK
   481  LSCMWEDIRW LRQSIPISSS SSTVLQTRQK MLAATAQLQN LLGTHNLGRV YYEPIKDRHG
   541  NILIVTIREV EMLYSFFNGK WMQISKLQSQ RKSLSTPEEP TALDILLITI QDILSYHKRS
   601  HQRLFPGLYL GYLKLCSSVD QIKVLVTQKL PNILCHVKIR ENNNISREEW EWIQKLSGSE
   661  SMESVDHTSD CPMQLFFYEL QMAVKALLQQ INIPLHQARN FRLYTQEVLE MGHNVSFLLL
   721  LPASDDVCTA PGQNNPYTPH SGFLNLPLQM FELVHFCSYR EKFISLYCRL SAVVELDSLN
   781  TQQSLREAIS DSEVAAAKQR HQQVLDFIQQ IDEVWREMRW IMDALQYARY KQPVSGLPIT
   841  KLIDPSDEQS LKKINSTSSS HIDCLPSPPP SPEMHRRKTV SDSQPCSDEE ACSEVFLPTN
   901  SDYDSSDALS PRDLDLVYLS SHDIAQQTLS GLSGSAPDVL QVHDVKTPLG PGQDPQGEGP
   961  NPDHSCAEFL HSLTLTGFTP KNHAKTVSGG RPPLGFLGKR KPGKHPHYGG FSRHHRWLRI
  1021  HSETQSLSLS EGIYTQHLSQ ACGLAQEPKE AKRAGPALDD PRGLTLAHAA SLPEERNSSL
  1081  QDARPSVRRL YVEPYAAAVV AQDEKPWASL SPPSGGRITL PSPTGPDVSQ EGPTASPMSE
  1141  ILSSML

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKFN1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 8.3 nTPM

Expression across tissuesHPA

Tissue

cervix: 8.3 nTPM
fallopian tube: 4.7 nTPM
skin: 1.8 nTPM
cerebral cortex: 1.6 nTPM
testis: 1.3 nTPM
epididymis: 0.9 nTPM

Single-cell type

respiratory ciliated cells: 918 nCPM
ependymal cells: 810 nCPM
fallopian tube ciliated cells: 409 nCPM
astrocytes: 340 nCPM
endometrial ciliated cells: 279 nCPM
adrenal medulla cells: 192 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

midbrain: 26 nTPM
hypothalamus: 22 nTPM
medulla oblongata: 19 nTPM
spinal cord: 18 nTPM
thalamus: 15 nTPM
pons: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.75
gnomAD pLI: 0
gnomAD missense Z: 0.56
DepMap mean gene effect: 0
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

behavioral fear response
equilibrioception
establishment of mitotic spindle orientation
locomotor rhythm
regulation of establishment of bipolar cell polarity

Cellular components

spindle

Protein domainsUniProt · Pfam · InterPro

Ankyrin repeat
Fibronectin type III
Immunoglobulin-like fold
Fibronectin type III superfamily
Ankyrin repeat-containing domain superfamily
Fibronectin type III domain
Ankyrin repeat and fibronectin type-III domain-containing protein 1

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKFN1 as an antibody target. Whether an autoantibody or antibody against ANKFN1 could matter depends on whether native ANKFN1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKFN1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKFN1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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