Seroatlas · Human Serome Atlas

ALG10

Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Also known as: AG10A_HUMAN, ALG10A, DIE2, FLJ14751

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5BKT4
Gene: ALG10
Ensembl: ENSG00000139133
Chromosome: 12
Canonical length: 473 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location: Endoplasmic reticulum

OverviewNCBI Gene

This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010]

Canonical amino-acid sequenceUniProt

473 residues, UniProt reviewed canonical sequence.

>Q5BKT4|ALG10
     1  MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE GHFSLSQWDP
    61  MITTLPGLYL VSIGVIKPAI WIFGWSEHVV CSIGMLRFVN LLFSVGNFYL LYLLFCKVQP
   121  RNKAASSIQR VLSTLTLAVF PTLYFFNFLY YTEAGSMFFT LFAYLMCLYG NHKTSAFLGF
   181  CGFMFRQTNI IWAVFCAGNV IAQKLTEAWK TELQKKEDRL PPIKGPFAEF RKILQFLLAY
   241  SMSFKNLSML LLLTWPYILL GFLFCAFVVV NGGIVIGDRS SHEACLHFPQ LFYFFSFTLF
   301  FSFPHLLSPS KIKTFLSLVW KRRILFFVVT LVSVFLVWKF TYAHKYLLAD NRHYTFYVWK
   361  RVFQRYETVK YLLVPAYIFA GWSIADSLKS KSIFWNLMFF ICLFTVIVPQ KLLEFRYFIL
   421  PYVIYRLNIP LPPTSRLICE LSCYAVVNFI TFFIFLNKTF QWPNSQDIQR FMW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALG10 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 11
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 3.8 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 3.8 nTPM
lymph node: 3.4 nTPM
thymus: 3.3 nTPM
fallopian tube: 3.2 nTPM
retina: 3 nTPM
liver: 2.7 nTPM

Single-cell type

early primary spermatocytes: 15 nCPM
megakaryocyte progenitors: 13 nCPM
adrenal medulla cells: 11 nCPM
erythrocyte progenitors: 10 nCPM
platelets: 9.8 nCPM
neutrophil progenitors: 8.9 nCPM

Immune cell

eosinophil: 1.7 nTPM
naive CD8 T-cell: 1.3 nTPM
T-reg: 1.2 nTPM
naive CD4 T-cell: 1.1 nTPM
classical monocyte: 1 nTPM
MAIT T-cell: 0.9 nTPM

Brain region

white matter: 8.8 nTPM
medulla oblongata: 8.5 nTPM
spinal cord: 8.1 nTPM
cerebellum: 8 nTPM
hypothalamus: 7.8 nTPM
pons: 7.6 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ALG10.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 87 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.2
gnomAD pLI: 0
gnomAD missense Z: -0.03
DepMap mean gene effect: -0.13
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

2-glucosyltransferase activity
dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ALG10 as an antibody target. Whether an autoantibody or antibody against ALG10 could matter depends on whether native ALG10 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ALG10 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ALG10 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...