ALDOB

Protein identityUniProt · HPA

UniProt accession

P05062

Gene

ALDOB

Ensembl

ENSG00000136872

Chromosome

9

Canonical length

364 aa

Protein class

Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins

Quaternary structure

Homotetramer

OverviewNCBI Gene

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

Canonical amino-acid sequenceUniProt

364 residues, UniProt reviewed canonical sequence.

>P05062|ALDOB
     1  MAHRFPALTQ EQKKELSEIA QSIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR
    61  EILFSVDSSI NQSIGGVILF HETLYQKDSQ GKLFRNILKE KGIVVGIKLD QGGAPLAGTN
   121  KETTIQGLDG LSERCAQYKK DGVDFGKWRA VLRIADQCPS SLAIQENANA LARYASICQQ
   181  NGLVPIVEPE VIPDGDHDLE HCQYVTEKVL AAVYKALNDH HVYLEGTLLK PNMVTAGHAC
   241  TKKYTPEQVA MATVTALHRT VPAAVPGICF LSGGMSEEDA TLNLNAINLC PLPKPWKLSF
   301  SYGRALQASA LAAWGGKAAN KEATQEAFMK RAMANCQAAK GQYVHTGSSG AASTQSLFTA
   361  CYTY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALDOB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.22

Highest tissue expression

10,467 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ALDOB.

Disease | AllUniProt

Conditions ALDOB is implicated in, by any mechanism.

• Hereditary fructose intolerance (HFI) MIM:229600

Disease | GeneticClinVar

139 pathogenic / likely-pathogenic of 598 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Hereditary fructosuria
• ALDOB-related disorder
• Inborn genetic diseases
• See cases
• Glycogen storage disease

Disease | ImmuneIEDB

Conditions an epitope on ALDOB was assayed in.

• allergic disease T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.1

gnomAD pLI

0

gnomAD missense Z

-1.42

DepMap mean gene effect

-0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• fructose 1,6-bisphosphate metabolic process
• fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
• fructose metabolic process
• gluconeogenesis
• glycolytic process
• negative regulation of pentose-phosphate shunt
• vacuolar proton-transporting V-type ATPase complex assembly

Molecular functions

• ATPase binding
• cytoskeletal protein binding
• fructose binding
• fructose-bisphosphate aldolase activity
• identical protein binding
• molecular adaptor activity
• fructose-1-phosphate aldolase activity

Cellular components

• centriolar satellite
• cytosol
• extracellular exosome
• microtubule organizing center

Protein domainsUniProt · Pfam · InterPro

• Fructose-bisphosphate aldolase, class-I
• Aldolase-type TIM barrel
• Fructose-bisphosphate aldolase class-I active site
• Fructose-bisphosphate aldolase class-I

KeywordsUniProt

• Acetylation
• Cytoplasm
• Cytoskeleton
• Glycolysis
• Lyase
• Phosphoprotein
• Schiff base

InteractionsUniProt · HPA

Protein binding partners of ALDOB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• BBS1
• BBS2
• BBS4
• BBS7
• ALDOA