Seroatlas · Human Serome Atlas

AIFM3

Apoptosis-inducing factor 3

Also known as: AIFL, AIFM3_HUMAN, FLJ30473

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96NN9
Gene: AIFM3
Ensembl: ENSG00000183773
Chromosome: 22
Canonical length: 605 aa
Protein class: Predicted intracellular proteins
Subcellular location: Mitochondria

OverviewNCBI Gene

Predicted to enable oxidoreductase activity, acting on NAD(P)H. Involved in execution phase of apoptosis. Located in cytosol; endoplasmic reticulum; and mitochondrial inner membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

605 residues, UniProt reviewed canonical sequence.

>Q96NN9|AIFM3
     1  MGGCFSKPKP VELKIEVVLP EKERGKEELS ASGKGSPRAY QGNGTARHFH TEERLSTPHP
    61  YPSPQDCVEA AVCHVKDLEN GQMREVELGW GKVLLVKDNG EFHALGHKCP HYGAPLVKGV
   121  LSRGRVRCPW HGACFNISTG DLEDFPGLDS LHKFQVKIEK EKVYVRASKQ ALQLQRRTKV
   181  MAKCISPSAG YSSSTNVLIV GAGAAGLVCA ETLRQEGFSD RIVLCTLDRH LPYDRPKLSK
   241  SLDTQPEQLA LRPKEFFRAY GIEVLTEAQV VTVDVRTKKV VFKDGFKLEY SKLLLAPGSS
   301  PKTLSCKGKE VENVFTIRTP EDANRVVRLA RGRNVVVVGA GFLGMEVAAY LTEKAHSVSV
   361  VELEETPFRR FLGERVGRAL MKMFENNRVK FYMQTEVSEL RGQEGKLKEV VLKSSKVVRA
   421  DVCVVGIGAV PATGFLRQSG IGLDSRGFIP VNKMMQTNVP GVFAAGDAVT FPLAWRNNRK
   481  VNIPHWQMAH AQGRVAAQNM LAQEAEMSTV PYLWTAMFGK SLRYAGYGEG FDDVIIQGDL
   541  EELKFVAFYT KGDEVIAVAS MNYDPIVSKV AEVLASGRAI RKREVELFVL HSKTGDMSWL
   601  TGKGS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AIFM3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 137 nTPM

Expression across tissuesHPA

Tissue

choroid plexus: 137 nTPM
cerebral cortex: 34 nTPM
cerebellum: 29 nTPM
amygdala: 22 nTPM
colon: 20 nTPM
midbrain: 20 nTPM

Single-cell type

astrocytes: 16 nCPM
retinal pigment epithelial cells: 13 nCPM
choroid plexus epithelial cells: 9.6 nCPM
colonocytes: 8.7 nCPM
enteric stem cells: 5.5 nCPM
bergmann glia: 5.2 nCPM

Immune cell

intermediate monocyte: 3 nTPM
myeloid DC: 1.9 nTPM
non-classical monocyte: 1.9 nTPM
classical monocyte: 1.1 nTPM
total PBMC: 0.5 nTPM
memory B-cell: 0.1 nTPM

Brain region

choroid plexus: 280 nTPM
thalamus: 141 nTPM
cerebral cortex: 100 nTPM
medulla oblongata: 90 nTPM
pons: 85 nTPM
midbrain: 84 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.99
gnomAD pLI: 0
gnomAD missense Z: 1
DepMap mean gene effect: -0.09
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

execution phase of apoptosis

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AIFM3 as an antibody target. Whether an autoantibody or antibody against AIFM3 could matter depends on whether native AIFM3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AIFM3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AIFM3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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