ADD1

Protein identityUniProt · HPA

UniProt accession

P35611

Gene

ADD1

Ensembl

ENSG00000087274

Chromosome

4

Canonical length

737 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Plasma membrane

OverviewNCBI Gene

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]

Canonical amino-acid sequenceUniProt

737 residues, UniProt reviewed canonical sequence.

>P35611|ADD1
     1  MNGDSRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN MMEQKKRVSM
    61  ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTT NVPNVYPAAP QGGMAALNMS
   121  LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA AFYRLADLFG WSQLIYNHIT TRVNSEQEHF
   181  LIVPFGLLYS EVTASSLVKI NLQGDIVDRG STNLGVNQAG FTLHSAIYAA RPDVKCVVHI
   241  HTPAGAAVSA MKCGLLPISP EALSLGEVAY HDYHGILVDE EEKVLIQKNL GPKSKVLILR
   301  NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLNPEKYK AKSRSPGSPV
   361  GEGTGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL REKSKKYSDV EVPASVTGYS
   421  FASDGDSGTC SPLRHSFQKQ QREKTRWLNS GRGDEASEEG QNGSSPKSKT KWTKEDGHRT
   481  STSAVPNLFV PLNTNPKEVQ EMRNKIREQN LQDIKTAGPQ SQVLCGVVMD RSLVQGELVT
   541  ASKAIIEKEY QPHVIVSTTG PNPFTTLTDR ELEEYRREVE RKQKGSEENL DEAREQKEKS
   601  PPDQPAVPHP PPSTPIKLEE DLVPEPTTGD DSDAATFKPT LPDLSPDEPS EALGFPMLEK
   661  EEEAHRPPSP TEAPTEASPE PAPDPAPVAE EAAPSAVEEG AAADPGSDGS PGKSPSKKKK
   721  KFRTPSFLKK SKKKSDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ADD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.51

Highest tissue expression

252 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ADD1.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 125 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Esophageal atresia/tracheoesophageal fistula

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.3

gnomAD pLI

0.99

gnomAD missense Z

0.8

DepMap mean gene effect

0.02

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• actin cytoskeleton organization
• actin filament bundle assembly
• barbed-end actin filament capping
• cellular response to calcium ion
• positive regulation of adherens junction organization
• positive regulation of establishment of endothelial barrier

Molecular functions

• actin binding
• actin filament binding
• cadherin binding
• calmodulin binding
• cytoskeletal anchor activity
• protein dimerization activity
• protein heterodimerization activity
• protein homodimerization activity
• RNA binding
• RNA polymerase II-specific DNA-binding transcription factor binding
• spectrin binding

Cellular components

• adherens junction
• cytoplasm
• cytoskeleton
• cytosol
• F-actin capping protein complex
• focal adhesion
• nuclear body
• nucleoplasm
• nucleus
• plasma membrane
• postsynaptic density

Protein domainsUniProt · Pfam · InterPro

• Class II aldolase/adducin N-terminal
• Class II aldolase/adducin N-terminal domain superfamily
• Aldolase class II Adducin subfamily
• Class II Aldolase and Adducin N-terminal domain

KeywordsUniProt

• Acetylation
• Actin-binding
• Calmodulin-binding
• Cell membrane
• Cytoplasm
• Cytoskeleton
• Membrane
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of ADD1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ACTB