Seroatlas · Human Serome Atlas

ADAT1

tRNA-specific adenosine deaminase 1

Also known as: ADAT1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9BUB4
Gene: ADAT1
Ensembl: ENSG00000065457
Chromosome: 16
Canonical length: 502 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

502 residues, UniProt reviewed canonical sequence.

>Q9BUB4|ADAT1
     1  MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT PDKPVQVTKE
    61  VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL LHQLQLAATL KEDSIFVPGT
   121  QKGVWKLRRD LIFVFFSSHT PCGDASIIPM LEFEDQPCCP VFRNWAHNSS VEASSNLEAP
   181  GNERKCEDPD SPVTKKMRLE PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL
   241  ATVTRIAPGS AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS
   301  DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ NVSALPKGFG
   361  VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW SAVPEQPLDV TANGFPQGTT
   421  KKTIGSLQAR SQISKVELFR SFQKLLSRIA RDKWPHSLRV QKLDTYQEYK EAASSYQEAW
   481  STLRKQVFGS WIRNPPDYHQ FK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ADAT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 21 nTPM

Expression across tissuesHPA

Tissue

testis: 21 nTPM
kidney: 18 nTPM
liver: 17 nTPM
spleen: 16 nTPM
parathyroid gland: 14 nTPM
adrenal gland: 13 nTPM

Single-cell type

late spermatids: 161 nCPM
early spermatids: 55 nCPM
late primary spermatocytes: 25 nCPM
neutrophils: 18 nCPM
platelets: 18 nCPM
renal collecting duct intercalated cells: 18 nCPM

Immune cell

eosinophil: 12 nTPM
MAIT T-cell: 11 nTPM
memory CD8 T-cell: 11 nTPM
NK-cell: 11 nTPM
non-classical monocyte: 10 nTPM
T-reg: 9.1 nTPM

Brain region

white matter: 22 nTPM
cerebral cortex: 20 nTPM
medulla oblongata: 20 nTPM
pons: 20 nTPM
thalamus: 20 nTPM
midbrain: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.19
gnomAD pLI: 0
gnomAD missense Z: -1.47
DepMap mean gene effect: -0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

tRNA processing

Molecular functions

metal ion binding
RNA binding
tRNA-specific adenosine deaminase activity
tRNA-specific adenosine-37 deaminase activity

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ADAT1 as an antibody target. Whether an autoantibody or antibody against ADAT1 could matter depends on whether native ADAT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ADAT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ADAT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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