ACOX3

Protein identityUniProt · HPA

UniProt accession

O15254

Gene

ACOX3

Ensembl

ENSG00000087008

Chromosome

4

Canonical length

700 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Peroxisomes

OverviewNCBI Gene

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

700 residues, UniProt reviewed canonical sequence.

>O15254|ACOX3
     1  MASTVEGGDT ALLPEFPRGP LDAYRARASF SWKELALFTE GEGMLRFKKT IFSALENDPL
    61  FARSPGADLS LEKYRELNFL RCKRIFEYDF LSVEDMFKSP LKVPALIQCL GMYDSSLAAK
   121  YLLHSLVFGS AVYSSGSERH LTYIQKIFRM EIFGCFALTE LSHGSNTKAI RTTAHYDPAT
   181  EEFIIHSPDF EAAKFWVGNM GKTATHAVVF AKLCVPGDQC HGLHPFIVQI RDPKTLLPMP
   241  GVMVGDIGKK LGQNGLDNGF AMFHKVRVPR QSLLNRMGDV TPEGTYVSPF KDVRQRFGAS
   301  LGSLSSGRVS IVSLAILNLK LAVAIALRFS ATRRQFGPTE EEEIPVLEYP MQQWRLLPYL
   361  AAVYALDHFS KSLFLDLVEL QRGLASGDRS ARQAELGREI HALASASKPL ASWTTQQGIQ
   421  ECREACGGHG YLAMNRLGVL RDDNDPNCTY EGDNNILLQQ TSNYLLGLLA HQVHDGACFR
   481  SPLKSVDFLD AYPGILDQKF EVSSVADCLD SAVALAAYKW LVCYLLRETY QKLNQEKRSG
   541  SSDFEARNKC QVSHGRPLAL AFVELTVVQR FHEHVHQPSV PPSLRAVLGR LSALYALWSL
   601  SRHAALLYRG GYFSGEQAGE VLESAVLALC SQLKDDAVAL VDVIAPPDFV LDSPIGRADG
   661  ELYKNLWGAV LQESKVLERA SWWPEFSVNK PVIGSLKSKL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACOX3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

25 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.06

gnomAD pLI

0

gnomAD missense Z

-0.44

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• fatty acid beta-oxidation using acyl-CoA oxidase

Molecular functions

• FAD binding
• fatty acid binding
• flavin adenine dinucleotide binding
• pristanoyl-CoA oxidase activity

Cellular components

• cytosol
• membrane
• peroxisomal matrix
• peroxisome

Protein domainsUniProt · Pfam · InterPro

• Acyl-CoA oxidase, C-terminal
• Acyl-CoA dehydrogenase/oxidase, middle domain
• Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily
• Acyl-CoA oxidase
• Peroxisomal acyl-coenzyme A oxidase
• Acyl-CoA dehydrogenase-like, C-terminal
• Acyl-CoA oxidase/dehydrogenase, middle domain superfamily
• Acyl-CoA oxidase, C-alpha1 domain
• Acyl-CoA oxidase
• Acyl-CoA dehydrogenase, middle domain
• Acyl-CoA oxidase, C-alpha1 domain

KeywordsUniProt

• Acetylation
• FAD
• Fatty acid metabolism
• Flavoprotein
• Lipid metabolism
• Oxidoreductase
• Peroxisome
• Phosphoprotein