ACOT8

Protein identityUniProt · HPA

UniProt accession

O14734

Gene

ACOT8

Ensembl

ENSG00000101473

Chromosome

20

Canonical length

319 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Vesicles,Mitochondria

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]

Canonical amino-acid sequenceUniProt

319 residues, UniProt reviewed canonical sequence.

>O14734|ACOT8
     1  MSSPQAPEDG QGCGDRGDPP GDLRSVLVTT VLNLEPLDED LFRGRHYWVP AKRLFGGQIV
    61  GQALVAAAKS VSEDVHVHSL HCYFVRAGDP KLPVLYQVER TRTGSSFSVR SVKAVQHGKP
   121  IFICQASFQQ AQPSPMQHQF SMPTVPPPEE LLDCETLIDQ YLRDPNLQKR YPLALNRIAA
   181  QEVPIEIKPV NPSPLSQLQR MEPKQMFWVR ARGYIGEGDM KMHCCVAAYI SDYAFLGTAL
   241  LPHQWQHKVH FMVSLDHSMW FHAPFRADHW MLYECESPWA GGSRGLVHGR LWRQDGVLAV
   301  TCAQEGVIRV KPQVSESKL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACOT8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

37 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1

gnomAD pLI

0

gnomAD missense Z

0.12

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• acyl-CoA metabolic process
• alpha-linolenic acid metabolic process
• bile acid biosynthetic process
• dicarboxylic acid catabolic process
• fatty acid beta-oxidation using acyl-CoA oxidase
• fatty acid catabolic process
• fatty acid derivative biosynthetic process
• long-chain fatty acid biosynthetic process
• negative regulation of glycoprotein biosynthetic process
• peroxisome fission
• unsaturated fatty acid biosynthetic process

Molecular functions

• acetyl-CoA hydrolase activity
• carboxylic ester hydrolase activity
• choloyl-CoA hydrolase activity
• fatty acyl-CoA hydrolase activity
• long-chain fatty acyl-CoA hydrolase activity
• medium-chain fatty acyl-CoA hydrolase activity
• succinyl-CoA hydrolase activity
• acetoacetyl-CoA hydrolase activity
• acyl-CoA hydrolase activity
• hydroxymethylglutaryl-CoA hydrolase activity

Cellular components

• cytosol
• peroxisomal matrix

Protein domainsUniProt · Pfam · InterPro

• HotDog domain superfamily
• Acyl-CoA thioesterase
• Acyl-CoA thioesterase, double hotdog domain
• Acyl-CoA thioesterase-like, N-terminal HotDog domain
• Acyl-CoA thioesterase-like, C-terminal domain
• Acyl-CoA thioesterase N-terminal domain
• Acyl-CoA thioesterase C-terminal domain

KeywordsUniProt

• Fatty acid metabolism
• Host-virus interaction
• Hydrolase
• Lipid metabolism
• Peroxisome
• Peroxisome biogenesis
• Serine esterase

InteractionsUniProt · HPA

Protein binding partners of ACOT8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PEX5