Seroatlas · Human Serome Atlas

ACOT4

Peroxisomal succinyl-coenzyme A thioesterase

Also known as: ACOT4_HUMAN, FLJ31235, PTE-Ib, PTE2B

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N9L9
Gene
ACOT4
Ensembl
ENSG00000177465
Chromosome
14
Canonical length
421 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

Enables fatty acyl-CoA hydrolase activity and succinyl-CoA hydrolase activity. Involved in carboxylic acid metabolic process and succinyl-CoA metabolic process. Located in peroxisome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

421 residues, UniProt reviewed canonical sequence.

>Q8N9L9|ACOT4
     1  MSATLILEPP GRCCWNEPVR IAVRGLAPEQ RVTLRASLRD EKGALFRAHA RYCADARGEL
    61  DLERAPALGG SFAGLEPMGL LWALEPEKPF WRFLKRDVQI PFVVELEVLD GHDPEPGRLL
   121  CQAQHERHFL PPGVRRQSVR AGRVRATLFL PPGPGPFPGI IDIFGIGGGL LEYRASLLAG
   181  HGFATLALAY YNFEDLPNNM DNISLEYFEE AVCYMLQHPQ VKGPGIGLLG ISLGADICLS
   241  MASFLKNVSA TVSINGSGIS GNTAINYKHS SIPPLGYDLR RIKVAFSGLV DIVDIRNALV
   301  GGYKNPSMIP IEKAQGPILL IVGQDDHNWR SELYAQTVSE RLQAHGKEKP QIICYPGTGH
   361  YIEPPYFPLC PASLHRLLNK HVIWGGEPRA HSKAQEDAWK QILAFFCKHL GGTQKTAVPK
   421  L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACOT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.23
Highest tissue expression
16 nTPM

Expression across tissuesHPA

Tissue

  • liver: 16 nTPM
  • kidney: 14 nTPM
  • adipose tissue: 4.6 nTPM
  • breast: 4.3 nTPM
  • duodenum: 4 nTPM
  • cervix: 3.6 nTPM

Single-cell type

  • smooth muscle cells: 2.6 nCPM
  • renal collecting duct intercalated cells: 2 nCPM
  • brain excitatory neurons: 1.8 nCPM
  • brain inhibitory neurons: 1.6 nCPM
  • other brain neurons: 1.6 nCPM
  • astrocytes: 1.3 nCPM

Immune cell

  • gdT-cell: 15 nTPM
  • basophil: 12 nTPM
  • naive CD8 T-cell: 8.6 nTPM
  • memory CD8 T-cell: 8.4 nTPM
  • MAIT T-cell: 7.3 nTPM
  • NK-cell: 7.2 nTPM

Brain region

  • cerebral cortex: 7.9 nTPM
  • cerebellum: 7.5 nTPM
  • basal ganglia: 6.8 nTPM
  • hippocampal formation: 6.4 nTPM
  • pons: 5.8 nTPM
  • white matter: 5.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.66
gnomAD pLI
0
gnomAD missense Z
0.11
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ACOT4 as an antibody target. Whether an autoantibody or antibody against ACOT4 could matter depends on whether native ACOT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ACOT4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ACOT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ACOT4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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