Seroatlas · Human Serome Atlas

ACOT1

Acyl-coenzyme A thioesterase 1

Also known as: ACH2, ACOT1_HUMAN, CTE-1, LACH2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86TX2
Gene: ACOT1
Ensembl: ENSG00000184227
Chromosome: 14
Canonical length: 421 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Mitochondria

OverviewNCBI Gene

Enables fatty acyl-CoA hydrolase activity. Involved in acyl-CoA metabolic process; long-chain fatty acid metabolic process; and very long-chain fatty acid metabolic process. Located in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

421 residues, UniProt reviewed canonical sequence.

>Q86TX2|ACOT1
     1  MAATLILEPA GRCCWDEPVR IAVRGLAPEQ PVTLRASLRD EKGALFQAHA RYRADTLGEL
    61  DLERAPALGG SFAGLEPMGL LWALEPEKPL VRLVKRDVRT PLAVELEVLD GHDPDPGRLL
   121  CRVRHERYFL PPGVRREPVR AGRVRGTLFL PPEPGPFPGI VDMFGTGGGL LEYRASLLAG
   181  KGFAVMALAY YNYEDLPKTM ETLHLEYFEE AVNYLLSHPE VKGPGVGLLG ISKGGELCLS
   241  MASFLKGITA AVVINGSVAN VGGTLRYKGE TLPPVGVNRN RIKVTKDGYA DIVDVLNSPL
   301  EGPDQKSFIP VERAESTFLF LVGQDDHNWK SEFYANEACK RLQAHGRRKP QIICYPETGH
   361  YIEPPYFPLC RASLHALVGS PIIWGGEPRA HAMAQVDAWK QLQTFFHKHL GGHEGTIPSK
   421  V

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACOT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.23
Highest tissue expression: 42 nTPM

Expression across tissuesHPA

Tissue

liver: 42 nTPM
tongue: 27 nTPM
kidney: 24 nTPM
smooth muscle: 20 nTPM
skeletal muscle: 19 nTPM
heart muscle: 18 nTPM

Single-cell type

epididymal principal cells: 67 nCPM
breast lactating cells: 37 nCPM
epididymal clear cells: 23 nCPM
breast myoepithelial cells: 22 nCPM
cardiomyocytes: 13 nCPM
adrenal medulla cells: 11 nCPM

Immune cell

T-reg: 5.9 nTPM
gdT-cell: 4.5 nTPM
memory CD4 T-cell: 4.4 nTPM
naive CD8 T-cell: 4.4 nTPM
myeloid DC: 4.1 nTPM
MAIT T-cell: 4 nTPM

Brain region

thalamus: 11 nTPM
amygdala: 8.7 nTPM
basal ganglia: 8.3 nTPM
midbrain: 8.3 nTPM
hippocampal formation: 8.1 nTPM
cerebral cortex: 8 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ACOT1.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 67 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Generalized hypotonia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.53
gnomAD pLI: 0
gnomAD missense Z: 0.85

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ACOT1 as an antibody target. Whether an autoantibody or antibody against ACOT1 could matter depends on whether native ACOT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ACOT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ACOT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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